Identification of Genes Involved in the Responses of Tangor (C. reticulata × C. sinensis) to Drought Stress

Figure 6

Quantitative real-time PCR (qRT-PCR) analyses of 18 differentially expressed transcripts for “Amakusa” tangor under drought treatments. Leaf tissues were sampled from plants on days 0, 3, 5, 8, and 11 after the start of water withdrawal. Three independent biological replications were performed. The relative expression level for stress-treated plants from 3 to 11 days was calculated as fold of control plants (0 day treatment) using the comparative method. All data were normalized to β-actin. The mean expression value was calculated for every transcript-derived fragment (TDF) with three replications. (Induced genes: TDF24, TDF26, TDF38, TDF43, and TDF82; upregulated genes: TDF33, TDF34, TDF60, TDF72, TDF92, TDF99, TDF109, and TDF120, TDF136; downregulated genes: TDF13 and TDF42; repressed gene: TDF64; inconsistent gene: TDF4.)