Research Article
Pharmacokinetic Properties of Adenosine Amine Congener in Cochlear Perilymph after Systemic Administration
Table 2
The LCMS/MS conditions and instrument settings used to assess ADAC concentrations in rat perilymph.
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Mobile Phase A: 4.5 mM ammonium formate pH 7.0. Mobile Phase B: 100% acetonitrile. Column: Phenomenex® Gemini 5 µm C18 110 Å, 150 × 3.00 mm S/N: 625787-3. Injection volume: 20 µL. Detection wavelength: 254 nm, bandwidth 20 nm. Source parameters: gas temp: 275°C; gas flow: 10 L/min; nebulizer: 25 psi; sheath gas temp: 350°C; sheath gas flow: 12 L/min. Capillary voltage: 2750 V (positive) and 3500 V (negative); nozzle voltage: 2000 V. Multiple reaction monitoring (MRM) transitions and compound-dependent parameters: precursor ion (m/z) Q1: 577.4; product ion (m/z) Q3: 445.4; collision energy: 20 V; fragmentation: 150 V; dwell time: 100 ms. Run time: 10 min; retention time ~2.5 min. Thermostat control: 5°C. |