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BioMed Research International
Volume 2017, Article ID 8206281, 7 pages
https://doi.org/10.1155/2017/8206281
Research Article

The Protective Effect of Chrysanthemum indicum Extract against Ankylosing Spondylitis in Mouse Models

1Infectious Immune Department of Rheumatism, Tianjin Hospital, Jiefangnan Road 406, Tianjin 300211, China
2Department of Rehabilitation Medicine, Tianjin Medical University, Tianjin 300052, China
3Department of Botany and Microbiology, Addiriyah Chair for Environmental Studies, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia

Correspondence should be addressed to Dongsheng Yu; moc.621@mdgnehsgnoduy

Received 12 December 2016; Accepted 15 January 2017; Published 2 February 2017

Academic Editor: Nigora Mukhamedova

Copyright © 2017 Mei Dong et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

In traditional Chinese and Korean homeopathic medicine, Chrysanthemum indicum Linné (Asteraceae) is a time-honored herb, prescribed for the resolution of symptoms associated with inflammatory and hypertensive conditions as well as those affecting the lungs and its associated structures. The goal of this work is to investigate the defensive role of Chrysanthemum indicum extract in fighting ankylosing spondylitis (AS) using mouse models, through which the manifestation and extent of the disease progression were measured with quantitative analysis of the intervertebral joints. Markers of inflammation as well as oxidative stress were also analysed. Western blot was used to quantify the levels of Nuclear Factor-κB (NF-κB) p65, Dickkopf-1 (DKK-1), and sclerostin (SOST). Consequently, the findings of this experiment demonstrated that AS in mice that were given Chrysanthemum indicum extract had lower level of TNF-α, IL-1β, and IL-6 () and increased level of catalase (CAT), glutathione peroxidase (GSH-PX), and superoxide dismutase (SOD) (). The results also revealed that Chrysanthemum indicum supplemented with diet contributed to a decrease in Nuclear Factor-κB (NF-κB) p65 protein expression () and higher levels of DKK-1 and SOST proteins (). Therefore, we concluded that the beneficial role of Chrysanthemum indicum in AS is manifested through downregulating oxidative stress, inhibiting inflammatory mediators and NF-κB, and increasing DKK-1 and SOST levels.