Neutrophil Extracellular Traps Are Pathogenic in Ventilator-Induced Lung Injury and Partially Dependent on TLR4

Figure 5

DNase attenuates lung injury and inflammation in mice. (a) The lung wet/dry weight ratio decreased partially in the DNase group compared with the vehicle group. (b)(c) The BALF total protein concentration and the neutrophil counts in the BALF all decreased in the DNase I pretreated mice (versus vehicle control). (d)(e) DNase I also reduced the BALF levels of IL-6 and TNF-α. (f) The lung injury score in the vehicle group was markedly higher than those in the DNase group. (g) Low (magnification 200x, scale bar = 40 μm) and high power (magnification 400x, scale bar = 20 μm) views of the lungs of the vehicle group and the DNase group. Hematoxylin and eosin stain. The vehicle group had much more inflammatory cell infiltration, alveolar septal thickening, pulmonary edema, and hemorrhage than the DNase group. compared to the vehicle group.

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