Plant Proteinase Inhibitor BbCI Modulates Lung Inflammatory Responses and Mechanic and Remodeling Alterations Induced by Elastase in Mice

Figure 2

Timeline of the experimental protocol. On the first day of protocol, animals received intratracheal instillation of elastase or vehicle. Two hours after the intranasal instillation, animals received an intraperitoneal injection of treatment [BbCI (SALBC and ELABC) or saline (ELA and SAL)]. On the fifteenth day, animals received the second dose of treatment [BbCI (SALBC and ELABC) or saline (ELA and SAL)]. On the twenty-first day, animals received the last dose of BbCI [BbCI (SALBC and ELABC) or saline (ELA and SAL)]. On the twenty-eighth day the animals were anesthetized and tracheostomized and the evaluation of the lung mechanics and the BAL was performed; the lungs were removed to do the morphometric analysis.