Upregulation of Mitf by Phenolic Compounds-Rich Cymbopogon schoenanthus Treatment Promotes Melanogenesis in B16 Melanoma Cells and Human Epidermal Melanocytes

Figure 4

Effect of Cymbopogon schoenanthus ethanol extract on the mRNA expression level of microphthalmia-associated transcription factor (Mitf) determined using TaqMan real-time quantitative PCR. B16 cells were cultured in 100 mm dish (3 × 10⁶ cells/dish) and treated without (CON) or with 1/1000 (v/v) C. schoenanthus ethanol extract (CYM), using 400 nm alpha-melanocyte-stimulating hormone (α-MSH) as a positive control, and incubated for 4 h after which RNA was extracted, and then reverse transcription PCR was carried out to obtain cDNAs that were used for real-time PCR (ABI 7500 Fast Real-time PCR system). Results represent the mean ± SD of three independent experiments. significant () difference between control and treated cells.