Identification of Sp1 as a Transcription Activator to Regulate Fibroblast Growth Factor 21 Gene Expression

Table 1

Sequences of primers and oligonucleotides used for generation of constructs, mutagenesis, and EMSA probes.

Primer/oligonucleotide	Sequence (5′ → 3′)
−1432F	GGGGTACCCAAAGGTTCTCCCACGGTTC
−906F	GGGGTACCACTGCTGATACAGCTCTCCT
−472F	GGGGTACCTCCCTCAGACTCAGGAGTGC
−99F	GGGGTACCAGGTTCCTGCCAAGTGTGTCA
−63F	GGGGTACCGGAGTGGGGAGGGCACGT
−20F	GGGGTACCTGGTATTTCTGCGTTCACCAG
CR	GGAAGATCTTGGGGCTCAGGCAAAGTGAA
mSp1-A F	TACCGGAGTGGttgaGGCACGTGGGCGGGCCT
mSp1-A R	AGGCCCGCCCACGTGCCtcaaCCACTCCGGTA
mSp1-B F	AGGGCACGTGttgaGGCCTGTCTG
mSp1-B R	CAGACAGGCCtcaaCACGTGCCCT
FGF21-probe	GCACGTGGGCGGGCCTGTCTG
Sp1C-probe	GTCAAGTTCGGGGAGGGGGATCAG

I and Bgl II restriction sites are underlined. CR, common reverse primer.
mutations are represented in lowercase letters.