Dexamethasone Modulates Nonvisual Opsins, Glucocorticoid Receptor, and Clock Genes in Danio rerio ZEM-2S Cells

Figure 2

Evidence of Opn4m2 immunoreactivity in D. rerio ZEM-2S cells. (a) Control preparation in which the primary antibody was omitted; (b) Opn4m2 labeling revealed by Cy3- secondary antibody in orange showing nuclear localization of the protein; (c) DAPI-labeled nuclei in blue; (d) merged (b) and (c) images. Photomicrographies were taken with Axiocam MRm camera (Zeiss) and pseudocolored with Axiovision Software (Zeiss). Scale bar 50 µm (200x magnification).

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