Dexamethasone Modulates Nonvisual Opsins, Glucocorticoid Receptor, and Clock Genes in Danio rerio ZEM-2S Cells

Figure 7

Time course of gr gene expression of D. rerio ZEM-2S cells exposed to LD (12 : 12) cycles and subject to (a) 2 medium changes (control) or 1 DEX pulse; (b) 6 medium changes (control) or 3 DEX pulses. Each value is the mean ( flasks), ±SEM, of transcripts normalized by ribosomal 18S RNA and expressed relative to 24 h average of gr after 2 medium changes. A significantly different from B and C from D demonstrated by one way ANOVA, followed by Tukey (). Asterisk means significantly different from the respective control at the same time point (, two way ANOVA followed by Bonferroni).

(a) 1 Dexamethasone Pulse

(b) 3 Dexamethasone Pulses