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BioMed Research International
Volume 2017, Article ID 8459385, 14 pages
Research Article

Dexamethasone Modulates Nonvisual Opsins, Glucocorticoid Receptor, and Clock Genes in Danio rerio ZEM-2S Cells

Department of Physiology, Institute of Biosciences, University of Sao Paulo, Sao Paulo, SP, Brazil

Correspondence should be addressed to Ana Maria de Lauro Castrucci;

Received 10 October 2016; Revised 19 March 2017; Accepted 22 March 2017; Published 14 May 2017

Academic Editor: Giuseppe Piccione

Copyright © 2017 Jennifer Caroline Sousa et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Danio rerio possess five melanopsin genes (Davis et al., 2011); among them, opn4m1 and opn4m2 were reported as the most expressed melanopsins in ZEM-2S cells (Ramos et al., 2014). The cellular compartments where Opn4m1 and Opn4m2 are expressed were identified here by immunocytochemistry. Surprisingly Opn4m2 was found in the nucleus when ZEM-2S cells were kept in DD conditions. So, we investigated the effects of white light pulse in promoting melanopsin translocation. Danio rerio ZEM-2S cells were kept for 3 days in DD condition and subject to 1 hour of white light pulse (650 lux) in the beginning of the 4th day. The incubation of ZEM-2S cells with anti-Opn4m2 at 1 : 100 dilution demonstrated that white light pulse did not altered Opn4m2 nuclear location. On the other hand, in mammalian melanoma cells, a short white light pulse (15 min) promotes melanopsin translocation from the nucleus to the cell membrane (de Assis et al., 2016). Davies, W. I. L. et al. (2011). Functional diversity of melanopsins and their global expression in the teleost retina. Cell. Mol. Life Sci. 68(24), 4115–4132. Ramos, B. C. R., Moraes, M. N. C. M., Poletini, M. O., Lima, L. H. R. G., and Castrucci, A. M. L. (2014) From blue light to clock genes in zebrafish ZEM-2S cells. PLoS ONE 9 (9), Article ID e106252. de Assis, L. V. M., Moraes, M. N., da Silveira Cruz-Machado, S., and Castrucci, A. M. L. (2016) The effect of white light on normal and malignant murine melanocytes: a link between opsins, clock genes, and melanogenesis. Biochim. Biophys. Acta 1863(6), 1119–1133.

  1. Supplementary Material