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BioMed Research International
Volume 2017 (2017), Article ID 8576404, 6 pages
https://doi.org/10.1155/2017/8576404
Research Article

Effect of Gamma Rays on Sophora davidii and Detection of DNA Polymorphism through ISSR Marker

1Guizhou Institute of Prataculture, Guiyang 550006, China
2College of Animal Science, Guizhou University, Guiyang 550025, China

Correspondence should be addressed to Lili Zhao; moc.361@8050_ililoahz

Received 14 November 2016; Accepted 28 February 2017; Published 22 May 2017

Academic Editor: Marco Bazzicalupo

Copyright © 2017 Puchang Wang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Sophora davidii (Franch.) Kom. ex Pavol is an important medicinal plant and a feeding scrub with ecological value. The effects of different gamma irradiation doses (20–140 Kr) on seed germination and seedling morphology were investigated in S. davidii, and intersimple sequence repeat (ISSR) markers were used to identify the DNA polymorphism among mutants. Significant variations were observed for seed germination, stem diameter, and number of branches per plant. The improved agronomic traits, such as stem diameter and number of branches per plant, were recorded at 80 Kr dose and 20 Kr dose for seed germination. ISSR analysis generated in total 183 scorable fragments, of which 94 (51.37%) were polymorphic. The percentage of polymorphism ranged from 14.29 to 93.33 with an average of 45.69%. Jaccard’s coefficients of dissimilarity varied from 0.6885 to 1.000, indicative of the level of genetic variation among the mutants. The constructed dendrogram grouped the entities into five clusters. Consequently, it was concluded that gamma rays irradiation of seeds generates a sufficient number of induced mutations and that ISSR analysis offered a useful molecular marker for the identification of mutants.