The Expression of AQP5 and UTs in the Sweat Glands of Uremic Patients

Table 2

The characteristics of primer sets and PCR conditions used to detect mRNA expression.

Gene	Sequence	Anneal temperature	Expected length
AQP5	F: 5′-CCACCTTGTCGGAATCTACTTC-3′	60.70°C	111 bp
	R: 5′-CCTACCCAGAAAACCCAGTGAG-3′	61.94°C
UT-A1	F: 5′-GACAGGTCTGCCATTGCCT-3′	60.00°C	540 bp
	R: 5′-GAGGACGCAGTTGTAGCTCCA-3′	62.09°C
UT-B1	F: 5′-CTCCCTGTATGTGCTATGTCCA-3′	60.07°C	142 bp
	R: 5′-TGTAATGTCCTGTGGCTGAAAG-3′	58.21°C
GAPDH	F: 5′-AGAAGGCTGGGGCTCATTTG-3′	59.85°C	258 bp
	R: 5′-AGGGGCCATCCACAGTCTTC-3′	61.90°C

F: forward primer. R: reverse primer.