Determination of Aflatoxin M1 and Chloramphenicol in Milk Based on Background Fluorescence Quenching Immunochromatographic Assay

Figure 3

Determination of optimal density of GNP-labeled antibody and concentration of coupled antibody of AFM1. AFM1 antibody was labeled by GNP solutions (pH 7.0) with a concentration of 0.30 μg/mL, 0.60 μg/mL, 0.90 μg/mL, and 1.20 μg/mL. The antibody with a concentration of 0.3 mg/mL (a), 0.5 mg/mL (b), and 1.0 mg/mL (c) was used to select the optimal density of GNP-labeled AFM1 antibody and concentration of AFM1 coupled antibody, defined as presence of significant difference between F₁/F₀ at four concentrations.

(a)

(b)

(c)