Figure 3: Cell reseeding and regeneration of dental pulp with decellularized pulp. Reseeded samples were transplanted subcutaneously in immunodeficient mice for 2 months, (a–c) H&E staining showed that pulp-like tissues were regenerated in recellularized matrix group, compared with natural pulp and blank control. (d–f) H&E staining showed the functional pulp was regenerated with a layer of mineral tissue regenerated (dashed line) between the dental pulp-like tissue and dentin interface, as well as a layer of polygonal cells (yellow arrow). (g) Quantitive analysis of pulp-like tissue regeneration area in control and recellularized matrix group. (h) Quantitive analysis of mineral tissue deposition area in natural pulp, blank control, and acellular pulp group. Values are mean ± SD, . One-way ANOVA was used to determine statistical significance, . D: dentin; P: pulp; Od: odontoblasts; scale bars: 50 μm.