Heterologous Secretory Expression and Characterization of Dimerized Bone Morphogenetic Protein 2 in Bacillus subtilis

Figure 1

Amplification of BMP2 monomer and dimer and construction of recombinant plasmids. (a) Amplified PCR product of hBMP2 resolved on 1% agarose gel showing band of BMP2 monomer at ~399 bp. Lane M, 1 kb DNA ladder; Lane 1, hBMP2 monomer. (b) Amplified PCR product of hBMP2 dimer showing covalently linked BMP2 dimer at ~780 bp. Lane M, 1 kb DNA ladder; Lane 1, hBMP2 dimer. (c) Recombinant plasmid pHT43-BMP2-M (8429 bp) showing position of the hBMP2 monomer along with restriction sites. (d) Recombinant plasmid pHT43-BMP2-D (8810 bp) showing position of hBMP2 dimer along with restriction sites.

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