Heterologous Secretory Expression and Characterization of Dimerized Bone Morphogenetic Protein 2 in Bacillus subtilis

Table 2

Sequence of hBMP2 monomer and dimer primers showing nucleotide and amplicon size (bp). The bold and underline sequences show restriction sites and His-tag (3′), respectively, incorporated in the primers.

Set of primers	Sequence (5′-3′)	Nucleotide (bp)	Amplicon size (bp)
FBMP2-M	CTATGCTTAGCAGGGGGATCCCAAGCCAAACACAAAC	37	399
	BamHI
RBMP2-M	TCTAGATTAATGGTGGTGGTGATGATGACCGCGACCGCGACACCCACAACCCTC	54
	XbaI
FBMP2-D	CTATGCTTAGCAGGGGGATCC	21	384
	BamHI
RBMP-L	AGATCTACTACCTGAACTACTGCGACACCCACAACCCTCC	40
	BglII
FBMP-L	AGATCTTCAGGTTCGTCTAGTGGTCAAGCCAAACACAAAC	40	402
	BglII
RBMP2-D	TCTAGATTAATGGTGGTGGTGATGATG	27
	XbaI