Immunogenicity and Cross Protection in Mice Afforded by Pandemic H1N1 Live Attenuated Influenza Vaccine Containing Wild-Type Nucleoprotein

Figure 2

Kinetics of virus replication in MDCK cells (MOI 0.01). Cell monolayers were infected with studied viruses at a multiplicity of infection (MOI) of 0.01 in triplicate and incubated at the permissive temperature (33°C). Culture supernatants were collected every 12 hours and stored at −70°C prior to titration by 50% tissue culture infective dose (TCID₅₀). Graphs show median titers with interquartile range (T-lines). Statistically significant differences between studied viruses are indicated with an asterisk (, Mann–Whitney test). Black circles: H1N1 LAIV (6 : 2) rg; grey squares: H1N1 LAIV (5 : 3) rg.