Therapy Effects of Wogonin on Ovarian Cancer Cells

Figure 3

Wogonin treatment induces apoptosis in A2780 ovarian cancer cells. (a) Representative images showing apoptosis detected with Annexin V-FITC and PI staining in A2780 cells. Percentage of the bottom right quadrant and the top right quadrant showing the representative events of early and late apoptosis, respectively. (b) Representative images of fluorescence microscopy analysis showing the effects of treatment with wogonin, MPP, and the combination on nuclei apoptosis and cell necrosis in A2780 cells. Cells were stained with Hoechst and PI, and images were taken under magnification of ×200. (c) The protein expression of Akt, Bcl-2,Bax, cytochrome C, caspase-3, and cleaved-caspase-3. β-Actin was included as a loading control.

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