Molecular Cloning, Bioinformatic Analysis, and Expression of Bombyx mori Lebocin 5 Gene Related to Beauveria bassiana Infection

Figure 3

Unrooted phylogenetic tree of BmLeb5 and other homologous proteins. The tree was constructed by the neighbor-joining method within the package MEGA 7. Bootstrap majority consensus values on 1,000 replicates are indicated at each branch point (%). The scale bar represents branch length (number of amino acids substitutions/100 residues). Accession numbers for sequences used in this alignment are as follows (GenBank ID): Bm5 (Bombyx mori, KX100575); Bm3 (Bombyx mori, NP_001119732.1); Bm4 (Bombyx mori, NP_001119731.1); Bm1/2 (Bombyx mori, NP_001037468.1); Se1 (Spodoptera exigua, AKJ54499.1); Gm (Galleria mellonella, ACQ99193.1); Ha (Helicoverpa armigera, ALT16900.1); At4 (Amyelois transitella, XP_013188814.1); Ap (Antheraea pernyi, ACB45567.1); MsB (Manduca sexta, ADE20197.1); MsC (Manduca sexta, ADE20198.1); Hv (Heliothis virescens, ACR78447.1); Pm1/2 (Papilio machaon, KPJ06168.1); Px1/2 (Papilio xuthus, KPI91575.1); Se2 (Spodoptera exigua, AKJ54500.1); Sr (Samia ricini, BAD84189.1); Dp (Danaus plexippus, EHJ64534.1); Px4 (Papilio xuthus, KPI91576.1); Pr (Pieris rapae, AEO21919.1); Am (Antheraea mylitta, ABG72704.1); Ob (Operophtera brumata, KOB78261.1); Ci (Chrysodeixis includens, AAS48093.1).