The Anti-Inflammatory Activity of Toonaciliatin K against Adjuvant Arthritis

Figure 1

The chemical structure, safety, and anti-inflammatory activities of toonaciliatin K on RAW264.7 cells. (a) The chemical structure of toonaciliatin K. (b) The MTT assay using toonaciliatin K at 24 h exposure on RAW264.7, HEK293, and L02 cells. For consequent assay, the RAW264.7 cells were treated with toonaciliatin K exposure (7, 14, or 28 μM) combined with LPS (10 ng/mL) for 12 h. The dexamethasone (Dex, 0.5 μg/mL) was used as positive. There were no LPS and toonaciliatin K in control group. (c) The mRNA expression levels of TNF-α, IL-6, and IL-1β. (d) The cytokines levels of TNF-α, IL-6, and IL-1β. (e) The mRNA levels of iNOS and COX-2. (f) The representative protein levels of iNOS and COX-2. (g) The quantification of protein levels of iNOS and COX-2. (h) The NO production levels. Statistical differences in different concentrations were considered significant at the levels of , , or . The statistical differences among LPS group and control group were considered significant at the levels of .

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