Laminin-521 Promotes Rat Bone Marrow Mesenchymal Stem Cell Sheet Formation on Light-Induced Cell Sheet Technology

Figure 4

Multilineage potential of rBMSC sheets. Alizarin-red staining was performed after osteogenic differentiation of cell sheets for 21 days (b). The control group was cultured in cell sheet nutrient solution (a). Oil Red O staining was performed after adipogenic induction of cell sheets for 21 days (e). As the control group, cell sheets were cultured in cell sheet nutrient solution (d). A small pellet formed after chondrogenic induction for 28 days (g). The pellet was cut into slices and stained with Alcian Blue (h). Relative mRNA expression levels in the osteogenic (c), adipogenic (f), chondrogenic (i) induced cell sheet group compared with that of the negative control group. . Scale bar: (a), (b), and (h): 200 μm; (d), (e): 100 μm.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)