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Figure 3: Apoptosis and necrosis of NB4 cells treated with esculetin and H2O2. Cells (5 × 105/ml) were treated with 100 M esculetin, 1 mM H2O2, or the combination of both, for the indicated times. Cells were then stained with FITC-conjugated annexin and PI and measured by flow cytometry. The results show one representative experiment out of three. Vertical axes: propidium iodide intensity (log scale); horizontal axes: annexin-FITC intensity (log scale).