Role of GntR Family Regulatory Gene SCO1678 in Gluconate Metabolism in Streptomyces coelicolor M145

Table 1

Bacterial strains and plasmids used in this work.

Strains or plasmids	Description	Source or reference
S. coelicolor M145	SCP1^−, SCP2^− derivative of A3(2); ACT and RED producer	[15]
S. coelicolor ΔgntR	SCO1678 knockout in M145	This work
S. coelicolor pKC-SCO1678	SCO1678 overexpression in M145	This work
S. coelicolor pKC1139	M145 with pKC1139 empty vector	This work
S. coelicolor pGUS	M145 carrying pGUS	This work
S. coelicolor pGUS-gntRp	M145 carrying pGUS-gntRp	This work
Escherichia coli DH5α	Routine cloning host	Life Technologies
E. coli ET12567 (pUZ8002)	Host for conjugative DNA transfer	[13]
E. coli BW25113 (pIJ790)	Host for recombineering experiments	[13]
E. coli BL21 (DE3) GOLD	Strain for recombinant protein production	Stratagene
pIJ790	ts-plasmid carrying genes for λ-RED recombination, CmlR	[13]
pLeere	Carrying acc(3)IV flanked by loxP-sites, apramycin and ampicillin resistances	Luzhetskyy
pKC1139	pSG5 ts-replicon; apramycin-resistant (AmR) shuttle vector	[15]
pKC-SCO1678	pKC1139 harboring SCO1678 with promoter region; SCO1678 overexpression, AmR	This work
pKC0702-SCO1678	pKC0702 harboring SCO1678 with 3-kb flanking region, HygR	This work
pKC0702-SCO1678::Am	SCO1678 knockout construct; sco1678::hyg (HygR and AmR)	This work
pGUS	Promoterless gusA-containing plasmid, apramycin resistance	[17]
pGUS-gntRp	pGUS with gntRp-gusA fusion, AmR	This work
pET28a	Protein expression vector, pET-system	Novagen
pET28a-SCO1678	Sco1678-6His protein expression	This work