Overexpression of MicroRNA-145 Ameliorates Astrocyte Injury by Targeting Aquaporin 4 in Cerebral Ischemic Stroke

Figure 1

miR-145 protected astrocytes from OGD-induced injury. (a) GFAP/DAPI staining of primary astrocytes (×200 magnification). (b) qRT-PCR detection of miR-145 expression in OGD primary cultured astrocytes or NC; U6 was used as the internal control ( versus control). (c) ELISA of LDH levels in astrocyte culture supernatant ( versus OGD; versus control). (d) Calcein-AM/PI staining cell health assay. PI-positive cells were dead cells; calcein-AM-positive cells were healthy cells ( versus OGD; versus control). (e) Flow cytometry assay of astrocyte apoptosis assay. Cells in the B2 and B4 quadrants represent apoptotic cells. (f) The percentage of apoptotic cells in each group. versus NC group; versus OGD group; versus control.

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