BioMed Research International / 2017 / Article / Fig 1

Research Article

Overexpression of MicroRNA-145 Ameliorates Astrocyte Injury by Targeting Aquaporin 4 in Cerebral Ischemic Stroke

Figure 1

miR-145 protected astrocytes from OGD-induced injury. (a) GFAP/DAPI staining of primary astrocytes (×200 magnification). (b) qRT-PCR detection of miR-145 expression in OGD primary cultured astrocytes or NC; U6 was used as the internal control ( versus control). (c) ELISA of LDH levels in astrocyte culture supernatant ( versus OGD; versus control). (d) Calcein-AM/PI staining cell health assay. PI-positive cells were dead cells; calcein-AM-positive cells were healthy cells ( versus OGD; versus control). (e) Flow cytometry assay of astrocyte apoptosis assay. Cells in the B2 and B4 quadrants represent apoptotic cells. (f) The percentage of apoptotic cells in each group. versus NC group; versus OGD group; versus control.

We are committed to sharing findings related to COVID-19 as quickly and safely as possible. Any author submitting a COVID-19 paper should notify us at to ensure their research is fast-tracked and made available on a preprint server as soon as possible. We will be providing unlimited waivers of publication charges for accepted articles related to COVID-19. Sign up here as a reviewer to help fast-track new submissions.