Overexpression of MicroRNA-145 Ameliorates Astrocyte Injury by Targeting Aquaporin 4 in Cerebral Ischemic Stroke

Figure 2

AQP4 was the target gene of miR-145. (a) Sequence alignment indicating nucleotide complementarity between miR-145 and the 3′ UTR of AQP4. (b) Schematic representation of miR-145-5p recognition site in AQP4 3′ untranslated region (UTR) and AQP4 mut-3′ UTR. (c) Bar graph represented normalized relative luciferase activity after cotransfection with miR-145-5p mimics (50 nM) in blank vector, wild type, and mutant type. versus vector, data are mean ± s.d., three independent experiments; versus vector. (d) Effect of miR-145 on AQP4 protein levels in primary astrocytes; β-actin was used as the internal control ( versus NC). (e) Effect of miR-145 on AQP4 protein levels in primary astrocytes with or without OGD; β-actin was used as the internal control. versus NC group; versus miR-145 mimics + OGD group; versus OGD miR-145 mimics + OGD group.

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