Overexpression of MicroRNA-145 Ameliorates Astrocyte Injury by Targeting Aquaporin 4 in Cerebral Ischemic Stroke

Figure 3

AQP4 knockdown inhibited OGD-induced primary astrocyte injury. (a) Real-time PCR detection of AQP4 mRNA levels in OGD astrocytes or NC; β-actin was used as the internal control ( versus control). (b, c) Western blots of AQP4 protein levels in OGD astrocytes or NC; β-actin was used as the internal control ( versus control). (d, e) Western blotting identification of AQP4 siRNA efficiency; β-actin was used as the internal control ( versus NC). (f) ELISA of LDH levels in astrocyte culture supernatant ( versus NC). (g) Calcein-AM/PI staining cell health assay. PI-positive cells are dead cells; calcein-AM-positive cells are healthy cells (, versus NC; ; versus NC + OGD; versus OGD). (h, i) Flow cytometry assay of astrocyte apoptosis. Cells in the B2 and B4 quadrants are apoptotic cells ( versus NC group; versus NC + OGD group). versus NC group; versus NC + OGD group.

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