Research Article

Cell Signaling Pathway in 12-O-Tetradecanoylphorbol-13-acetate-Induced LCN2 Gene Transcription in Esophageal Squamous Cell Carcinoma

Figure 4

Analyses of LCN2 mRNA levels in EC109 treated with MEK (mitogen-activated protein kinase/ERK kinase) specific inhibitors. Total RNA was extracted from EC109 cells treated with MEK specific inhibitors U0126 and PD98059, respectively. Real-time PCR assay was carried out with the Rotor-Gene 6000 system. All PCRs were performed in triplicate. The relative LCN2 mRNAs levels were normalized to that of β-actin mRNA and then shown to be relative to that of TPA treating control group. Differences were considered statistically significant at ().