Cell Signaling Pathway in 12-O-Tetradecanoylphorbol-13-acetate-Induced LCN2 Gene Transcription in Esophageal Squamous Cell Carcinoma

Figure 6

The analyses of ERK1/2 and p-ERK1/2 in EC109 cells treated with MEK specific inhibitor U0126. Total protein was collected from EC109 cells pretreated with MEK specific inhibitor (U0126) for 1 h and then treated with TPA for another 6 h, 12 h, and 24 h. ERK1/2 and p-ERK1/2 detection were, respectively, detected by western blotting.