Research Article

Vascular Tissue Engineering: Effects of Integrating Collagen into a PCL Based Nanofiber Material

Figure 5

Endothelial cells produce and secrete VEGF. The effect of gelatine-coated wells, PCL, and PCL/Coll nanofiber scaffolds on VEGF secretion of T17b eEPCs was determined from cell culture supernatants after 24, 48, and 72 h. (a) 24 h results show advantages of PCL/Coll 25% scaffolds over pure PCL and bsGel-TB groups (b). After 72 h, measured VEGF concentrations were significantly higher in PCL/Coll 25%, 50%, and 75% than in the pure PCL control group. Statistically significant differences between the different groups are indicated for and .