Evaluation of Epigallocatechin-3-gallate Modified Collagen Membrane and Concerns on Schwann Cells

Figure 4

Immunofluorescence staining of Krox-20 (red) and DAPI (blue) in RSC96 cells on collagen membranes treated with 0% (a), 0.0064% (b), 0.064% (c), and 0.64% (d) EGCG. Compared to the collagen membranes in the control group (a), the collagen membranes treated with different concentrations of EGCG showed increased expression of Krox-20 (f). (e–g) Results of Western blot assay, indicating that EGCG-treated collagen induced Krox-20 expression and decreased ROS levels in RSC96 cells. (h) After gray level difference analysis of Krox-20, the gray levels of 0.64% E and 0.064% E were significantly higher than that of the control group. ; , one-way ANOVA with Tukey’s multiple comparison test.

(a) Control

(b) 0.0064% E-Col

(c) 0.064% E-Col

(d) 0.64% E-Col

(e)

(f)

(g)

(h)