(a)
(b)
(c)
Figure 6: The effect of rs760057865 (C>T) on the expression level of miR-103a-1. MiR-103a-1 and pre-miR-67 were cotransfected into HEK293 cells, and the expression level of the mature miR-67 was used to calibrate the expression level of miR-103a-1. (a) Relative expression level of pri-miR-103a-1 (NC, ; NC + WT67, ; WT103 + WT67, ; MU103 + WT67, ; p, NC + WT67/NC, 0.0328; WT103 + WT67/NC, 0.0043; MU103 + WT67/NC, 0.0086). (b) Relative expression level of pre-miR-103a-1 (NC, ; NC + WT67, ; WT103 + WT67, ; MU103 + WT67, ; p, NC + WT67/NC, 0.0345; WT103 + WT67/NC, 0.0016; MU103 + WT67/NC, 0.0058). (c) Relative expression level of miR-103a (NC, ; NC + WT67, ; WT103 + WT67, ; MU103 + WT67, ; p, NC + WT67/NC, 0.6695; WT103 + WT67/NC, 0.3438; MU103 + WT67/NC, 0.0030). ; . NC, negative control, empty plasmid; NC + WT67, control; WT67: cel-miR-67-5p mirVana®miRNA mimic (Thermo Fisher number MC22484); WT103 + WT67, wild 103 + 67, WT-Hsa-miR-103a-1; MU103 + WT67, mutation 103 + 67, MT-Hsa-miR-103a-1.