Molecular Mechanism for the Regulation of Microcystin Toxicity to Protein Phosphatase 1 by Glutathione Conjugation Pathway

Table 1

Preparation and purification information for MCLR-GSH and MCRR-GSH.

Conjugation products	Eluted time	Concentration	Total volume	Purity
MCLR-GSH	12.54 min	≈1285 μmol/	μL	98.3%
MCRR-GSH	8.43 min	≈1094 μmol/L	μL	98.7%

a: collection time was set for 0.5 min (±0.25 min around the eluted time).
b: with 200 μmol/L MCLR (MCRR) serving as the inner standard for quantification and assuming MCLR and MCLR-GSH (MCRR and MCRR-GSH) had approximate protonated efficiencies.
c: purity was directed calculated by MS signals and defined as MC-GSH/(MC + MC-GSH.