Downregulation of Profilin-1 Expression Attenuates Cardiomyocytes Hypertrophy and Apoptosis Induced by Advanced Glycation End Products in H9c2 Cells

Figure 1

The changes of PFN-1 expression in H9c2 cells induced by AGEs. (a) Dose study: PFN-1 protein and mRNA expression in H9c2 cells incubated with different doses of AGEs for 24 hours. (b) Time study: PFN-1 protein and mRNA expression in H9c2 cells incubated with AGEs at a dose of 200 μg/ml for different times. (c) The effect of different inhibitors on AGEs-induced (200 μg/ml, 24 hours) PFN-1 protein and mRNA expression. (d) Left: representative images show PFN-1 Adv-shRNA adenovirus transfection probed by GFP (magnification: 20x; scale bar: 200 μm). Middle and right: the effect of PFN-1 Adv-shRNA on PFN-1 protein and mRNA expression incubated with or without AGEs (200 μg/ml, 24 hours). Data was expressed as mean ± SD, –6. versus control; versus AGEs; versus Adv-Control, versus A + Adv-Control.

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