Time- and Dose-Dependent Effects of 17 Beta-Estradiol on Short-Term, Real-Time Proliferation and Gene Expression in Porcine Granulosa Cells
Cell proliferation index (CI) of porcine follicular granulosa cells cultivated for 168 h with acute E2 treatment during the logarithmic increase of proliferation. Porcine follicular granulosa cells were recovered from pubertal gilts and treated with collagenase for 10 min at 38.5°C. The cells were immediately transferred into an E-Plate 48 of real-time cell analyzer (RTCA, Roche-Applied Science, GmbH, Penzberg, Germany). The experiment consisted of eight replicates involving the cultivation of the same population of collected cells. Similarly, the PI was evaluated at three different time periods, that is, 0–168 h (Figure 9(a)), 85–97 h (Figure 9(b)), and 93–97 h (Figure 9(c)). The porcine GCs were treated with 1.8 and 3.6 μM E2 at 96 h of culture (Figure 9(b)). The differences were considered to be significant at the level of and . A, B, and C represent triplicates (three samples of the same population of collected cells).
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