MicroRNA-29b Contributes to Collagens Imbalance in Human Osteoarthritic and Dedifferentiated Articular Chondrocytes

Figure 5

Effect of a stable miR-29b overexpression on ATDC5 cells phenotype during chondrogenesis. Undifferentiated cells were transfected with pcDNA3.1-pre-miR-29b or a negative control (pcDNA3.1-empty) and then cultured for 14 days in chondrogenic conditions. (a) Representative pictures of Sirius red staining of monolayer (left) or micromass (right) cultures. (b) COL1A1, COL1A2, COL2A1, and COL10A1 mRNA levels normalized on RPS29 mRNA determined by RT-quantitative PCR. (c) Expression of miR-29b mRNA relative to miR-21 (invariant endogenous control) determined by the method. Data are presented as relative levels where control cells were allocated arbitrarily the value 1. Bars show the mean value ± SEM of 3 independent experiments, each of them being run in triplicate. versus control.

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