BioMed Research International

BioMed Research International / 2018 / Article

Review Article | Open Access

Volume 2018 |Article ID 1012789 | 9 pages |

Origin of the Autophagosome Membrane in Mammals

Academic Editor: Terry K. Smith
Received09 May 2018
Revised18 Aug 2018
Accepted03 Sep 2018
Published24 Sep 2018


Autophagy begins with the nucleation of phagophores, which then expand to give rise to the double-membrane autophagosomes. Autophagosomes ultimately fuse with lysosomes, where the cytosolic cargoes are degraded. Accumulation of autophagosomes is a hallmark of autophagy and neurodegenerative disorders including Alzheimer’s and Huntington’s disease. In recent years, the sources of autophagosome membrane have attracted a great deal of interests, even so, the membrane donors for autophagosomes are still under debate. In this review, we describe the probable sources of autophagosome membrane.

1. Introduction

Macroautophagy (henceforth known as autophagy) is a nonselective “self-eating” process that maintains cellular homeostasis, manages stress responses, and controls large proteins and cytoplasmic components quality by eliminating defective or superfluous molecules/structures such as misfolded proteins, damaged mitochondria, excessive peroxisomes, ribosomes, and invading pathogens. Autophagy, which can be induced by exogenous stimulations, such as nutrient starvation, endoplasmic reticulum (ER) stress, rapamycin, vitamin D3, and IFN-γ treatment, provides a source of nutrition and emergy during periods of stress to promote healthy cell homeostasis and synaptic function. Dysfunction of autophagy is a misregulated process in various neurodegenerative diseases, different types of cancer, autoimmune diseases, and uncontrolled infections characterized by the accumulation of protein aggregates, degradation of intracellular pathogens, and clearance of aging organelles, including Alzheimer’s disease, Huntington’s disease, Parkinson’s disease, and amyotrophic lateral sclerosis.

The function of autophagy relies on the formation of double- or multimembrane vesicles named autophagosome (AP), which plays a key role in cell homeostasis through engulf cargo including damaged mitochondria (mitophagy) and protein aggregates. The activity of autophagy is modulated primarily by the size and number of APs. The production/accumulation of APs subsequently unfuse to lysosomes (or accumulation of APs) directly induces cellular toxicity under the condition of various stress conditions, such as oxidation and toxic protein aggregation, and this process may be implicated in the pathogenesis of neurodegenerative diseases, tumorigenesis, and infections, among others. The acidic pH and enzymatic action of hydrolases within the lysosome lead to the breakdown of the internal membranes of APs as well as the APs’ contents. So far, however, the origins of the autophagosomal membrane and the molecular mechanisms of AP formation are still unknown.

Here, we organize and summarize the papers in this issue according to four focus areas: morphology, formation, function, and source of AP.

2. Morphology of Autophagosome

As the double- or multimembrane organelle, the AP in the cytoplasm is a hallmark and the key initial event of autophagy. Sometimes double-membrane structure which contains part of cytoplasmic components also can be judged as AP. The size and number of APs may be separately regulated by different subgroups of autophagy-related genes (ATGs) proteins and the members of the ATG8/ light chain 3 (LC3) protein family. This makes sense considering that modulating AP size may affect primarily cargo selectivity, while regulating the number of APs could be carried out to regulate mostly autophagy flux. In mammals, the time of the AP formation takes 5-10 min [1]. The time courses of starvation decide the extent of AP expansion. The amount of ATG9 protein correlates with the numbers of autophagic bodies; that is, ATG9 levels determine the number of APs. Nitrogen-starvation induces APs range from 300 to 900 nm in diameter, larger than most other vesicles in the cell. During nonselective autophagy, premature closure of the phagophore results in smaller AP.

Another study has found that the size of AP may also depend on specific cargo [2], which can range from proteins to intracellular bacteria (0.06 to 0.2 μm) [3]. The size and formation of APs are regulated by the steady-state level of microtubule-associated protein LC3 [4], but the regulatory mechanism of this protein is not understood. The size of APs is likely determined by distinct autophagic steps. Frist, APs can expand by the addition of membrane to form isolation membranes during the early stage of autophagy. Second, APs may grow by fusion with endosomes and lysosomes at the late stage of autophagy, though endosomal/lysosomal fusion is not sufficient for proper autophagosomal growth. Besides, the extent of AP expansion is mainly dependent on the time course of induction condition, such as starvation. The reduction of the cellular level of ATG8, which anchored to the surface of APs, results in smaller AP compared with a wild-type strain, but the number of AP is the same as that of the wild type. Meanwhile, the AP is a highly dynamic organelle and its proteome differs if cells are under the conditions of starvation or basal macroautophagy when blocked with concanamycin A.

3. Formation of Autophagosome

AP formation is a complex series of discrete events which is mediated and controlled by a large number of proteins, but the process is poorly understood. Essentially, APs are formed by the induction, expansion (the phagophore/isolation membrane and the omegasome), vesicle completion (the AP), fusion (the amphisome), and degradation (the autolysosome/lysosome) [5], as shown in Figure 1. In the brain, AP biogenesis occurs distally in a constitutive process at the neurite tip which is far away from the nucleus in a microtubule- and dynein-dynactin motor complex-dependent manner. Various stress conditions, such as starvation, oxidation, and toxic protein aggregation, can accelerate the biogenesis of APs and degradation of lysosome. A mature AP is generated when the isolation membrane closes, and then the mature APs fuse with the vacuole/lysosome, where the contents are degraded and the products recycled to the cytosol for reuse. Nearly 40 ATGs have been identified and most of them are conserved across higher eukaryotes, but only parts of ATGs are directly associated with mammalian AP biogenesis, as shown in Table 1. The homotypic fusion of ATG16-positive and LC3-negative AP precursors is a critical regulatory step in AP biogenesis. As the conjugated form of LC3, LC3-II is associated with the outer of the autophagosomal membranes following completion and remains with the AP until fusion with the lysosomes [6]. As an aside, elevated levels of LC3-II generally correlate with the accumulation of APs in the cell but so not indicate an increase in AP biogenesis.

ATG ProteinsFeaturesFunction in AP

ULK1/2 [7]ATG1Serine/threonine kinase; form a complex with mATG13, FIP200 and ATG101; phosphorylated by mTORC1 and AMPK kinaseslate stage of AP biogenesis

ATG2A/B [8]ATG2Interacts with ATG18; associates to autophagosomal membranes through lipid binding and independently from ATG9closure of the AP membrane, late stage of AP biogenesis

ATG3[9]ATG3E2-like enzymefacilitates LC3/GABARAP lipidation in highly curved membranes; curvature and maturation of AP biogenesis

ATG4A-D [1]ATG4cysteine protease; phosphorylation by ATG1initial stage of phagophore formation

ATG5[10]ATG5conjugated by ATG12elongation of the isolation membranes, the AP-formation marker

vacuolar protein sorting (Vps)-30
conjugated by PI3KC3 and ULKintervene at every major step in autophagic pathways, from autophagosome formation, to autophagosome/endosome maturation

ATG7ATG7autophagy-related E1-like enzymeelongation of the AP membranes

LC3A/B/C, GABARAP, GATE-16, GABARAPL1/2/3 [4]ATG8ubiquitin-like protein; conjugates to phosphatidylethanolamine (PE)determines the size of AP; induce membrane tethering and fusion; expansion and closure of phagophores

ATG9L1 [11]ATG9transmembrane autophagy-related proteininitial stage of AP formation, generate the isolation membrane

ATG10ATG10E2-like enzyme; catalyze or facilitate ATG5-12 conjugationpromotes autophagolysosome formation

ATG11Scaffold Proteinregulates autophagosome-vacuole fusion

ATG12 [12]ATG12ubiquitin-like molecules; conjugates to ATG5elongation and maturation of the phagophore membrane

ATG13Phosphorylated by (m)TORC1later stage of autophagosome maturation

ATG14(L)/Barkor [14]ATG14autophagy-specific subunitfusion of APs to endolysosomes, regulates autophagosome nucleation; the preautophagosome/autophagosome marker

ATG16L1/2 [15]ATG16 conjugated by ATG12 and ATG5, E3‐Ubiquitin ligase‐like enzymeelongation of AP membrane

WIPI1/2/3/4 [16]ATG18PtdIns(3)P-binding proteinrecycle of membrane proteins from the vacuole to the late endosome

ATG19 [17]ATG19contains multiple ATG8 binding sitesserves as cargo receptor and directly interacts with ATG8 on the isolation membrane

ATG20 [18]sorting nexinrequired for efficient autophagy and membrane tubulation

ATG21PtdIns(3)P-binding protein, only detected at endosomes facilitates the recruitment of Atg8-PE to the site of autophagosome formation

_ATG23peripheral membrane proteinfacilitates Atg9 trafficking

SNX4ATG24 [19]a member of the BAR domain family of proteinsinhibits the number of APs

ATG27 [20]transmembrane proteinretrieval of Atg9 from the vacuole

RB1CC1/FIP200ATG17PI3P binding effector
ATG29Ternary complex with Atg17 and Atg31Atg29-Atg31-Atg17 complex [21], formation a dimer with two crescents for fusion into the expanding phagophore
ATG31Ternary complex with Atg17 and Atg29

ATG32 [22]outer mitochondrial membrane proteinessential for the initiation of mitophagy; facilitates mitochondrial capture in phagophores

ATG101Interacts with Atg13; maintains ULK1 basal phosphorylationinteracts with the ULK1 complex via direct binding to ATG13 to induce the formation of AP

There are reports that the ULK1/ATG1-ATG13-FIP200-ATG101 protein kinase complex, the VPS (vacuolar protein sorting) 34 (VPS34) complex, the ATG9 trafficking system, ATG5-ATG12-ATG16L1 complex, the two ubiquitin-like proteins ATG12 and ATG8/LC3, and their conjugation systems all have been found which lead to formation and expansion of the phagophore, which eventually seals to form the complete AP. Meanwhile, AP formation is highly inducible. Amino acid can induce autophagy and the protein kinase complex TORC1/mTORC1 suppresses AP formation in nutrient-rich conditions. Actin protein also is necessary for starvation-mediated autophagy through the Arp2/3 complex and WHAMM and actin depolymerization participates in the formation of APs at a very early stage rather than in the maturation steps. In addition, the deconjugation of ATG8–phosphatidylethanolamine (PE) is also required for efficient AP biogenesis for optimal phagophore expansion.

4. Function of Autophagosome

Autophagy is an evolutionarily conserved cellular process to maintain energy homeostasis. As the marker of autophagy, the dynamics and functions of APs remain robust in the mouse model of neurodegenerative disease, but AP flux is not increased even as protein accumulates along the axon [23]. Currently, stimulation of APs synthesis is often used to enhance autophagy to alleviate aggregation toxicity of protein in neurodegeneration and aging. Retrograde transport of APs might play a role in neuronal signaling processes, promoting neuronal morphological complexity and preventing neurodegeneration. Autophagy can promote infection by picornaviruses, such as poliovirus and coxsackieviruses, just because APs provide sites for replication. As the double-membrane vesicles, the compositions of the outer and inner AP membranes seem to be quite different [24]. So, there are different roles of different membranes: the inner autophagosomal membrane in charge of cargo sequestration and the outer autophagosomal membrane in charge of fusion with the lysosomal membrane.

Meanwhile two characteristics make APs a unique type of cellular transport carrier. First, two lipid bilayers surround the cargo and second, these giant vesicles generally have an average diameter of approximately 700 nm, which can further expand to accommodate large structures such as cellular organelles and bacteria [25]. But accumulation of APs causes cytotoxicity; especially in certain stress conditions the excessive APs subsequently unfused to lysosomes directly induce cellular toxicity independent of apoptosis and necroptosis, and this process may be implicated that AP is a hallmark of neurodegenerative disorders including Alzheimer’s and Huntington’s disease or amyotrophic lateral sclerosis [26, 27].

5. Source of Autophagosome Membrane

Because autophagy is an unselective bulk degradation pathway, the specific membrane origin of all APs remains obscure, though morphological features of APs are basically common to conventional and alternative autophagy. Different from yeast and plant cells, there is no preautophagosomal structure (PAS) in mammalian cells. The endoplasmic reticulum exit sites (ERES), mitochondria, ER-mitochondria contact sites, ER-Golgi intermediate compartment (ERGIC), Golgi apparatus, and plasma membrane (PM) have been suggested to supply lipids to the growing isolation membrane in mammalian cells, but the exact mechanism mediating this process remains ambiguity. It is possible that there are different membrane sources dependent on the cell type, growth conditions, physiological conditions, and so on, as shown in Table 2.

Origin Parts of probableInduction conditionDifferent stages of autophagosomesContribution

Mitochondriaouter mitochondrial membrane [32]serum, or serum and amino acid deprivation [32]phagophore expansionthe isolation membrane (also called the phagophore) expansion [1]

Endoplasmic reticulum the rough endoplasmic reticulum [33], subdomain of the ER termed the omegasomeamino acid starvation [34], fasted animalsearly stages of autophagosome formation [35]phagophore expansion, elongation of isolation membranes [36]

Golgitrans-Golgi network,TGNnitrogen starvation or fasted animalsearly stages of autophagosome formation [37]phagophore formation [38] and expansion, maturation of autophagosomes [39]

Plasma membraneATG16L vesicle, lipids of the plasma membraneamino acid and serum starvation, or nitrogen starvation[40]early stages of autophagosome formation [41] the formation of early autophagic precursors [42]

ER–mitochondria contact sitethe mitochondria-associated ER membrane (MAM)rapamycin and Torin 1 [43]uncertainphagophore expansion

ERGIC ERGIC-enriched membranenutrient starvation [43]generate an early autophagosomal membrane precursor [44]trigger phagophore formation

Recycling endosomesmembrane lipidsnutrient starvation or rich mediumearly stages of autophagosome formationsupply membrane lipids for autophagosome formation

6. ER-Mitochondria Contact Sites [28]

ER-mitochondria contact sites have gained a lot of attention recently. In 1952, Wilhelm Bernhard first reported the ER-mitochondria contact sites in rat liver by electron micrographs [29]. The ER-mitochondria contact sites are essential for several key cellular processes, such as calcium homeostasis, lipid metabolism, autophagy regulation, mitochondrial dynamics [30], cell survival to energy metabolism, and protein folding [31]. The correct maintenance of the ER-mitochondria interface is a critical part of the autophagic process; meanwhile the emergence of phagophores and the sites of contact between the ER and mitochondria have surprising correlation. Upon starvation, components of the autophagy-specific class III PI3K (ATG14L, Vps34, Beclin1, and Vps15) all accumulated in the mitochondria-associated membranes (MAMs) fraction and probably recruited to ER-mitochondria contact sites upon autophagy induction, and the autophagosome-formation marker ATG5 also localizes at the ER-mitochondria contact site until AP formation is complete. And AP formation is significantly suppressed in mitofusin 2-knockout cells, in which the ER-mitochondria contact sites are disrupted [32].

7. Endoplasmic Reticulum Exit Sites

As the largest organelle in the cell, the ER is in close proximity with other endomembrane compartments because of its membrane lipids and proteins synthesis and outward transports, establishing membrane-membrane contact sites (MCS) that facilitate signaling events, modulate dynamic organelle processes, and exchange the lipids and ions. ERES, the specialized regions of the ER where COPII transport vesicles are generated, are thought to be spatially, physically, and functionally linked to APs. Early autophagic structures tightly associate with the ER membrane due to the presence of omegasome subdomain positive for double FYVE-containing protein 1 (DFCP1), a phosphatidyl-inositol-3-phosphate (PI3P) binding protein; meanwhile the omegasome isolation membrane and AP phagophore probably are all derived from the ER [34]. Sandra Maday [45] also thought that APs are generated at DFCP1-positive subdomains of the ER in the distal end of the axon, distinct from ER exit sites in primary neurons. Studies have found that [46] lysosome membrane-associated protein-2 (LAMP-2), as a heavily glycosylated type-1 membrane protein, must be critical for translocating syntaxin-17 (STX17) to autophagosomal membranes. Sanchez-Wandelmer et al. [47] have found that ERES are core elements in the formation of isolation membranes and ER associates with the extension of isolation membranes or phagophore in mammalian cells by electron tomography. However, contradictory data emerged indicating that only 30% of all APs are associated with the ER or specialized regions of the ER [36].

8. Mitochondria

The mitochondria are the most important organelle in determining fundamental metabolic activities, iron and calcium homeostasis, and signal transduction of various cellular pathways. Mitochondrial dysfunction and dysregulation may lead to many human maladies, including cardiovascular diseases, neurodegenerative disease, and cancer. A number of reports suggest that [32] there is a connection between mitochondrial outer membrane lipids, proteins, and AP formation. It is suggested that the outer mitochondrial membrane donates a flow of lipids and membrane proteins to the AP. The early autophagy protein ATG5 and the autophagosomal marker LC3 translocate to puncta localized on the outer membrane of mitochondria following starvation, suggesting that mitochondria plays a central role in starvation-induced autophagy. Meanwhile, researches have found mitochondrial membrane donation to AP formation in both basal (in the presence of serum and vehicle) and drug-induced autophagy in human breast cancer cell line, other than being engulfed by the forming AP [48]. In other studies the authors also show that the connection between ER and mitochondria is crucial because in its absence, starvation-induced APs are not formed [32]. The counterargument is that mitochondria have nothing to the source of AP membranes, because mammalian ATG9 is found only to localize to the trans-Golgi network (TGN) and late endosomes, but not to mitochondria [49], while ATG9-containing compartments are a source of membranes for the formation and/or expansion of APs.

9. Golgi Apparatus

The Golgi apparatus is a major glycosylation site involved in protein and lipid synthesis, modification, and secretion. The Golgi apparatus is proposed to be a pivotal membrane source for the mammal AP formation. This result is first observed in developing invertebrate fat body cells by Locke and Collins in 1965. More researches have found that Golgi complex contributes to an early stage of autophagy [50]. At cell telophase, the Golgi structures in APs are again observed to be distributed at the cell periphery when Golgi apparatus is known to reassemble. Based on these, they proposed that Golgi apparatus is a membrane source for autophagosomal growth. As the only transmembrane ATG protein, ATG9 has been associated with the Golgi apparatus and may be involved in providing membrane for AP formation [51]. However, others argued that mammalian ATG9 (mATG9 and ATG9L1) is seen to associate with many other compartments, including recycling endosomes, early endosomes, and late endosomes [37]. It is possible that these organelles all participate in AP formation.

10. Plasma Membrane

The PM, which forms the barrier between the cytoplasm and the environment [52], plays critical roles in promoting virulence through mediating secretion of virulence factors, endocytosis, cell wall synthesis, and invasive hyphal morphogenesis. Meanwhile, proteins in the PM also mediate nutrient transport and sense pH, osmolarity, nutrients, and other factors in the extracellular environment. The ability of PM to contribute the AP formation may be particularly important in time of increasing autophagy in mammalian cells. The PM’s large surface area might act as a massive membrane store that allows cells to experience cycles of AP synthesis at much higher rates than under basic conditions without compromising other processes. ATGs and membranes that are necessary for AP formation are originated from PM [47]. Claudia Puri et al. found that ATG16L1 associates with clathrin-coated pits, and after internalization and uncoating, the ATG16L1-associated PM becomes associated with phagophore precursors, which mature into phagophores and then APs [53].

11. Recycling Endosomes

In mammalian cells, the endosomal system is extremely dynamic and generates several structurally and functionally distinct compartments, namely, early/recycling endosomes (REs), late endosomes, and lysosomes. The identity of endosomes is ensured by the specific localization of regulators. REs consist of a tubular network that emerges from vacuolar sorting endosomes and diverts cargoes toward the cell surface, the Golgi, or lysosome-related organelles. REs are also implicated in AP formation. mATG9, trafficking from the PM to the REs, is essential for the initiation and progression of autophagy. A. Orsi and his research team have found that mATG9-positive structures interact dynamically with phagophores and APs. TBC1D14, a Tre-2/Bub2/Cdc16 (TBC) domain protein, regulates Tfn receptor- (TfnR-) positive REs, which are required for AP formation. As a positive regulator of AP formation, the membrane remodeling protein sorting nexin18 (SNX18) is required for regulating ATG9A trafficking from REs and formation of ATG16L1- and WIPI2-positive AP precursor membranes [54]. ATG16L1 and mATG9-positive vesicles are present in the same sites on RE, which can reduce membrane egress from the REs to increase the formation of AP. These all indicate that REs probably are membrane donator for SNX18-mediated AP biogenesis.

12. Endoplasmic Reticulum-Golgi Intermediate Compartment

ERGIC structures may move from ERES to the Golgi apparatus by tracking on microtubules. Membrane traffic between the ER and the Golgi is bidirectional and occurs via similar mechanisms as other MAMs. Recently, ERGIC, a membrane compartment between the ER and Golgi for cargo sorting and recycling, is proposed as another membrane source for the phagophore. At the ER-Golgi interface, coat protein complex I (COPI) vesicle buds and facilitates retrograde transport from the Golgi and ERGIC, while COPII vesicles may be the precursor of the phagophore membrane. As the donor membrane, the ERGIC is a sorting station undergoing dynamic membrane exchange with COPI and COPII vesicles [55], the later of which are supposed to a source of membrane for APs at the ERGIC. Liang Ge et al. [44] also found that generation of COPII vesicles from the ERGIC could thus be a special event for autophagy-related membrane mobilization induced by starvation-induced remodeling of ERES. Drugs that disrupt ERGIC also suppressed LC3 conjugation and LC3 dot formation.

13. ER-Plasma Membrane Contact Sites (ER-PMcs)

ER-PMcs are also mobilized for AP biogenesis. At present researches have identified some functions of ER-PMcs, for example, regulate Ca2+ signaling [56], and conserve lipid homeostasis. The research team of Nascimbeni AC revealed that ER-PMcs can tether to extended synaptotagmins (E-Syts) proteins, which are essential for autophagy-associated phosphatidyl-inositol-3-phosphate (PI3P) synthesis at the cortical ER membrane, and then adjust the mammal AP biogenesis [57].

According to other literatures, ATG9-containing cytoplasmic vesicles (ATG9 vesicles) [58], ER- lipid droplet (LD) contact site [59], and COPII vesicles [11]s are also considered sources of membrane to build the AP. Still others think that the ATG9-associated membranes do not fuse with APs and only regulate autophagy because of its transient structural or catalytic functions [60].

14. Conclusion

Lowering the accumulation of APs may be a treatment option for neurodegenerative diseases with protein aggregates, so make sure where does the AP membrane come from and how is it formed? In recent years, these questions have attracted a great deal of interests. ER, mitochondria, Golgi complex, and the plasma membrane all have been proposed as the sources of autophagosomal membranes. Of course, the origin of AP membrane is probability multisources, which has been proved by more and more researches. The diverse origins of AP membrane interact each other. These different conclusions reached by the different laboratories could be in part due to different experimental approaches and techniques used in the various laboratories. And the relative contribution of each source under any one set of conditions remains to be determined. Although distinct sources of AP membranes have been proposed, it is not clear to what extent they may be mutually exclusive or whether they may coalesce and cooperate. A lot of research is needed.

Conflicts of Interest

The authors declare that they have no conflicts of interest.


The authors gratefully acknowledge the financial support from Beijing Natural Science Foundation Proposed Program (no. 7162169) and National Natural Science Foundation of China (no. 81173383, 81573819).


  1. J. Sánchez-Wandelmer and F. Reggiori, “Atg4 in autophagosome biogenesis,” Oncotarget , vol. 8, no. 65, pp. 108290-108291, 2017. View at: Google Scholar
  2. N. Mizushima, Y. Ohsumi, and T. Yoshimori, “Autophagosome formation in mammalian cells,” Cell Structure and Function, vol. 27, no. 6, pp. 421–429, 2002. View at: Publisher Site | Google Scholar
  3. B. Ravikumar, S. Sarkar, J. E. Davies et al., “Regulation of mammalian autophagy in physiology and pathophysiology,” Physiological Reviews, vol. 90, pp. 1383–1435, 2010. View at: Publisher Site | Google Scholar
  4. Z. Xie, U. Nair, and D. J. Klionsky, “Atg8 controls phagophore expansion during autophagosome formation,” Molecular Biology of the Cell (MBoC), vol. 19, no. 8, pp. 3290–3298, 2008. View at: Publisher Site | Google Scholar
  5. T. Yoshimori and T. Noda, “Toward unraveling membrane biogenesis in mammalian autophagy,” Current Opinion in Cell Biology, vol. 20, no. 4, pp. 401–407, 2008. View at: Publisher Site | Google Scholar
  6. K. E. Eng, M. D. Panas, D. Murphy, G. B. K. Hedestam, and G. M. McInerney, “Accumulation of autophagosomes in Semliki Forest virus-infected cells is dependent on expression of the viral glycoproteins,” Journal of Virology, vol. 86, no. 10, pp. 5674–5685, 2012. View at: Publisher Site | Google Scholar
  7. M. Kijanska and M. Peter, “Atg1 kinase regulates early and late steps during autophagy,” Autophagy, vol. 9, no. 2, pp. 249–251, 2013. View at: Publisher Site | Google Scholar
  8. A. K. G. Velikkakath, T. Nishimura, E. Oita, N. Ishihara, and N. Mizushima, “Mammalian Atg2 proteins are essential for autophagosome formation and important for regulation of size and distribution of lipid droplets,” Molecular Biology of the Cell (MBoC), vol. 23, no. 5, pp. 896–909, 2012. View at: Publisher Site | Google Scholar
  9. T. Hanada, N. N. Noda, Y. Satomi et al., “The Atg12-Atg5 conjugate has a novel E3-like activity for protein lipidation in autophagy,” The Journal of Biological Chemistry, vol. 282, no. 52, pp. 37298–37302, 2007. View at: Publisher Site | Google Scholar
  10. N. Mizushima, A. Yamamoto, M. Hatano et al., “Dissection of autophagosome formation using Apg5-deficient mouse embryonic stem cells,” The Journal of Cell Biology, vol. 152, no. 4, pp. 657–668, 2001. View at: Publisher Site | Google Scholar
  11. S. Davis, J. Wang, M. Zhu et al., “Sec24 phosphorylation regulates autophagosome abundance during nutrient deprivation,” eLife, vol. 5, no. 2016, 2016. View at: Google Scholar
  12. W. H. Meijer, I. J. van der Klei, M. Veenhuis, and J. A. K. W. Kiel, “ATG genes involved in non-selective autophagy are conserved from yeast to man, but the selective Cvt and pexophagy pathways also require organism-specific genes,” Autophagy, vol. 3, no. 2, pp. 106–116, 2007. View at: Publisher Site | Google Scholar
  13. H. Weidberg, E. Shvets, T. Shpilka, F. Shimron, V. Shinder, and Z. Elazar, “LC3 and GATE-16/GABARAP subfamilies are both essential yet act differently in autophagosome biogenesis,” EMBO Journal, vol. 29, no. 11, pp. 1792–1802, 2010. View at: Publisher Site | Google Scholar
  14. J. Diao, R. Liu, Y. Rong et al., “ATG14 promotes membrane tethering and fusion of autophagosomes to endolysosomes,” Nature, vol. 520, no. 7548, pp. 563–566, 2015. View at: Publisher Site | Google Scholar
  15. J. H. Kim, S. B. Hong, J. K. Lee et al., “Insights into autophagosome maturation revealed by the structures of ATG5 with its interacting partners,” Autophagy, vol. 11, no. 1, pp. 75–87, 2015. View at: Publisher Site | Google Scholar
  16. S. K. Dove, R. C. Piper, R. K. McEwen et al., “Svp1p defines a family of phosphatidylinositol 3,5-bisphosphate effectors,” EMBO Journal, vol. 23, no. 9, pp. 1922–1933, 2004. View at: Publisher Site | Google Scholar
  17. C. Abert, G. Kontaxis, and S. Martens, “Accessory interaction motifs in the Atg19 cargo receptor enable strong binding to the clustered ubiquitin-related Atg8 protein,” The Journal of Biological Chemistry, vol. 291, no. 36, pp. 18799–18808, 2016. View at: Publisher Site | Google Scholar
  18. H. Popelka, A. Damasio, J. E. Hinshaw, D. J. Klionsky, and M. J. Ragusa, “Structure and function of yeast Atg20, a sorting nexin that facilitates autophagy induction,” Proceedings of the National Acadamy of Sciences of the United States of America, vol. 114, no. 47, pp. E10112–E10121, 2017. View at: Publisher Site | Google Scholar
  19. A. Brennand, E. Rico, D. J. Rigden, P. Van Der Smissen, P. J. Courtoy, and P. A. M. Michels, “ATG24 represses autophagy and differentiation and is essential for homeostasy of the flagellar pocket in trypanosoma brucei,” PLoS ONE, vol. 10, no. 6, 2015. View at: Google Scholar
  20. V. A. Segarra, D. R. Boettner, and S. K. Lemmon, “Atg27 tyrosine sorting motif is important for its trafficking and Atg9 localization,” Traffic, vol. 16, no. 4, pp. 365–378, 2015. View at: Publisher Site | Google Scholar
  21. K. Mao, L. H. Chew, Y. Inoue-Aono et al., “Atg29 phosphorylation regulates coordination of the Atg17-Atg31-Atg29 complex with the Atg11 scaffold during autophagy initiation,” Proceedings of the National Acadamy of Sciences of the United States of America, vol. 110, no. 31, pp. E2875–E2884, 2013. View at: Publisher Site | Google Scholar
  22. X. Xia, S. Katzenell, E. F. Reinhart, K. M. Bauer, M. Pellegrini, and M. J. Ragusa, “A pseudo-receiver domain in Atg32 is required for mitophagy,” Autophagy, vol. 14, no. 9, pp. 1620–1628, 2018. View at: Publisher Site | Google Scholar
  23. S. Maday, K. E. Wallace, and E. L. F. Holzbaur, “Autophagosomes initiate distally and mature during transport toward the cell soma in primary neurons,” The Journal of Cell Biology, vol. 196, no. 4, pp. 407–417, 2012. View at: Publisher Site | Google Scholar
  24. N. Mizushima, “Autophagy: process and function,” Genes & Development, vol. 21, no. 22, pp. 2861–2873, 2007. View at: Publisher Site | Google Scholar
  25. Z. Xie and D. J. Klionsky, “Autophagosome formation: core machinery and adaptations,” Nature Cell Biology, vol. 9, no. 10, pp. 1102–1109, 2007. View at: Publisher Site | Google Scholar
  26. R. W. Button, S. L. Roberts, T. L. Willis, C. Oliver Hanemann, and S. Luo, “Accumulation of autophagosomes confers cytotoxicity,” The Journal of Biological Chemistry, vol. 292, no. 33, pp. 13599–13614, 2017. View at: Publisher Site | Google Scholar
  27. F. M. Menzies, A. Fleming, and D. C. Rubinsztein, “Compromised autophagy and neurodegenerative diseases,” Nature Reviews Neuroscience, vol. 16, no. 6, pp. 345–357, 2015. View at: Publisher Site | Google Scholar
  28. M. S. Herrera-Cruz and T. Simmen, “Of yeast, mice and men: MAMs come in two flavors,” Biology Direct, vol. 12, no. 1, 2017. View at: Google Scholar
  29. W. Bernhard, F. Haguenau, A. Gautier, and C. Oberling, “Submicroscopical structure of cytoplasmic basophils in the liver, pancreas and salivary gland study of ultrafine slices by electron microscope,” Z Zellforsch Mikrosk Anat, vol. 37, no. 3, pp. 281–300, 1952. View at: Google Scholar
  30. M. Krols, G. van Isterdael, B. Asselbergh et al., “Mitochondria-associated membranes as hubs for neurodegeneration,” Acta Neuropathologica, vol. 131, no. 4, pp. 505–523, 2016. View at: Publisher Site | Google Scholar
  31. M. Hamasaki, N. Furuta, A. Matsuda et al., “Autophagosomes form at ER-mitochondria contact sites,” Nature, vol. 495, no. 7441, pp. 389–393, 2013. View at: Publisher Site | Google Scholar
  32. D. W. Hailey, A. S. Rambold, P. Satpute-Krishnan et al., “Mitochondria supply membranes for autophagosome biogenesis during starvation,” Cell, vol. 141, no. 4, pp. 656–667, 2010. View at: Publisher Site | Google Scholar
  33. W. A. Dunn Jr., “Studies on the mechanisms of autophagy: Formation of the autophagic vacuole,” The Journal of Cell Biology, vol. 110, no. 6, pp. 1923–1933, 1990. View at: Publisher Site | Google Scholar
  34. P. Ylä-Anttila, H. Vihinen, E. Jokitalo, and E.-L. Eskelinen, “3D tomography reveals connections between the phagophore and endoplasmic reticulum,” Autophagy, vol. 5, no. 8, pp. 1180–1185, 2009. View at: Publisher Site | Google Scholar
  35. S. G. Pfisterer, D. Bakula, T. Frickey et al., “Lipid droplet and early autophagosomal membrane targeting of Atg2A and Atg14L in human tumor cells,” Journal of Lipid Research, vol. 55, no. 7, pp. 1267–1278, 2014. View at: Publisher Site | Google Scholar
  36. M. Hayashi-Nishino, N. Fujita, T. Noda, A. Yamaguchi, T. Yoshimori, and A. Yamamoto, “A subdomain of the endoplasmic reticulum forms a cradle for autophagosome formation,” Nature Cell Biology, vol. 11, no. 12, pp. 1433–1437, 2009. View at: Publisher Site | Google Scholar
  37. A. Orsi, M. Razi, H. C. Dooley et al., “Dynamic and transient interactions of Atg9 with autophagosomes, but not membrane integration, are required for autophagy,” Molecular Biology of the Cell (MBoC), vol. 23, no. 10, pp. 1860–1873, 2012. View at: Publisher Site | Google Scholar
  38. H. Yamamoto, S. Kakuta, T. M. Watanabe et al., “Atg9 vesicles are an important membrane source during early steps of autophagosome formation,” The Journal of Cell Biology, vol. 198, no. 2, pp. 219–233, 2012. View at: Publisher Site | Google Scholar
  39. Y. Takahashi, C. L. Meyerkord, T. Hori et al., “Bif-1 regulates Atg9 trafficking by mediating the fission of Golgi membranes during autophagy,” Autophagy, vol. 7, no. 1, pp. 61–73, 2011. View at: Publisher Site | Google Scholar
  40. U. Nair, A. Jotwani, J. Geng et al., “SNARE proteins are required for macroautophagy,” Cell, vol. 146, no. 2, pp. 290–302, 2011. View at: Publisher Site | Google Scholar
  41. B. Ravikumar, K. Moreau, L. Jahreiss, C. Puri, and D. C. Rubinsztein, “Plasma membrane contributes to the formation of pre-autophagosomal structures,” Nature Cell Biology, vol. 12, no. 8, pp. 747–757, 2010. View at: Publisher Site | Google Scholar
  42. B. Ravikumar, K. Moreau, and D. C. Rubinsztein, “Plasma membrane helps autophagosomes grow,” Autophagy, vol. 6, no. 8, pp. 1184–1186, 2010. View at: Publisher Site | Google Scholar
  43. L. Ge, M. Zhang, S. J. Kenny et al., “Remodeling of ER-exit sites initiates a membrane supply pathway for autophagosome biogenesis,” EMBO Reports, vol. 18, no. 9, pp. 1586–1603, 2017. View at: Publisher Site | Google Scholar
  44. L. Ge, L. Wilz, and R. Schekman, “Biogenesis of autophagosomal precursors for LC3 lipidation from the ER-Golgi intermediate compartment,” Autophagy, vol. 11, no. 12, pp. 2372–2374, 2015. View at: Publisher Site | Google Scholar
  45. S. Maday and E. L. F. Holzbaur, “Autophagosome biogenesis in primary neurons follows an ordered and spatially regulated pathway,” Developmental Cell, vol. 30, no. 1, pp. 71–85, 2014. View at: Publisher Site | Google Scholar
  46. V. Hubert, A. Peschel, B. Langer, M. Gröger, A. Rees, and R. Kain, “LAMP-2 is required for incorporating syntaxin-17 into autophagosomes and for their fusion with lysosomes,” Biology Open, vol. 5, no. 10, pp. 1516–1529, 2016. View at: Publisher Site | Google Scholar
  47. J. Sanchez-Wandelmer, N. T. Ktistakis, and F. Reggiori, “ERES: Sites for autophagosome biogenesis and maturation?” Journal of Cell Science, vol. 128, no. 2, pp. 185–192, 2015. View at: Publisher Site | Google Scholar
  48. K. L. Cook, D. R. Soto-Pantoja, M. Abu-Asab, P. A. G. Clarke, D. D. Roberts, and R. Clarke, “Mitochondria directly donate their membrane to form autophagosomes during a novel mechanism of parkin-associated mitophagy,” Cell & Bioscience, vol. 4, article 16, 2014. View at: Publisher Site | Google Scholar
  49. A. R. J. Young, E. Y. W. Chan, X. W. Hu et al., “Starvation and ULK1-dependent cycling of mammalian Atg9 between the TGN and endosomes,” Journal of Cell Science, vol. 119, no. 18, pp. 3888–3900, 2006. View at: Publisher Site | Google Scholar
  50. J. Geng, U. Nair, K. Yasumura-Yorimitsu, and D. J. Klionsky, “Post-golgi sec proteins are required for autophagy in Saccharomyces cerevisiae,” Molecular Biology of the Cell (MBoC), vol. 21, no. 13, pp. 2257–2269, 2010. View at: Publisher Site | Google Scholar
  51. A. S. Coutts and N. B. La Thangue, “Regulation of actin nucleation and autophagosome formation,” Cellular and Molecular Life Sciences, vol. 73, no. 17, pp. 3249–3263, 2016. View at: Publisher Site | Google Scholar
  52. A. Horn and J. K. Jaiswal, “Cellular mechanisms and signals that coordinate plasma membrane repair,” Cellular and Molecular Life Sciences, 2018. View at: Publisher Site | Google Scholar
  53. C. Puri, M. Renna, C. F. Bento, K. Moreau, and D. C. Rubinsztein, “Diverse autophagosome membrane sources coalesce in recycling endosomes,” Cell, vol. 154, no. 6, pp. 1285–1299, 2013. View at: Publisher Site | Google Scholar
  54. K. Søreng, M. J. Munson, C. A. Lamb et al., “SNX18 regulates ATG9A trafficking from recycling endosomes by recruiting Dynamin-2,” EMBO Reports, vol. 19, no. 4, 2018. View at: Google Scholar
  55. F. Brandizzi and C. Barlowe, “Organization of the ER-Golgi interface for membrane traffic control,” Nature Reviews Molecular Cell Biology, vol. 14, no. 6, pp. 382–392, 2013. View at: Publisher Site | Google Scholar
  56. M. M. Wu, J. Buchanan, R. M. Luik, and R. S. Lewis, “Ca2+ store depletion causes STIM1 to accumulate in ER regions closely associated with the plasma membrane,” The Journal of Cell Biology, vol. 174, no. 6, pp. 803–813, 2006. View at: Publisher Site | Google Scholar
  57. A. C. Nascimbeni, F. Giordano, N. Dupont et al., “ER–plasma membrane contact sites contribute to autophagosome biogenesis by regulation of local PI3P synthesis,” EMBO Journal, vol. 36, no. 14, pp. 2018–2033, 2017. View at: Publisher Site | Google Scholar
  58. S. W. Suzuki, H. Yamamoto, Y. Oikawa et al., “Atg13 HORMA domain recruits Atg9 vesicles during autophagosome formation,” Proceedings of the National Acadamy of Sciences of the United States of America, vol. 112, no. 11, pp. 3350–3355, 2015. View at: Publisher Site | Google Scholar
  59. T. Shpilka, E. Welter, N. Borovsky et al., “Lipid droplets and their component triglycerides and steryl esters regulate autophagosome biogenesis,” EMBO Journal, vol. 34, no. 16, pp. 2117–2131, 2015. View at: Publisher Site | Google Scholar
  60. D. C. Rubinsztein, T. Shpilka, and Z. Elazar, “Mechanisms of autophagosome biogenesis,” Current Biology, vol. 22, no. 1, pp. R29–R34, 2012. View at: Publisher Site | Google Scholar

Copyright © 2018 Yun Wei et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

More related articles

2270 Views | 515 Downloads | 13 Citations
 PDF  Download Citation  Citation
 Download other formatsMore
 Order printed copiesOrder

Related articles

We are committed to sharing findings related to COVID-19 as quickly and safely as possible. Any author submitting a COVID-19 paper should notify us at to ensure their research is fast-tracked and made available on a preprint server as soon as possible. We will be providing unlimited waivers of publication charges for accepted articles related to COVID-19. Sign up here as a reviewer to help fast-track new submissions.