Functional Polymorphisms in IRAKs Are Related to Hepatocellular Carcinoma Risk in Chinese Population

Figure 2

The proliferation rate of rs4251545 (IRAK4 p.Ala428Thr) among different liver cell lines. (a) 100 ng plasmid of IRAK4 428Ala or IRAK4 428Thr (rs4251545) was transfected into HepG2.2.15 cells, respectively, then the proliferation rate was measured at 12 h, 24 h, 36 h, 48 h, and 72 h, individually. Empty vector was set as the negative control. The dot, square, and triangle referred to control, IRAK4 428Ala, and IRAK4 428Thr, respectively. (b) mRNA of HBV was detected after transfection of IRAK4 428Ala or 428Thr in HepG2.2.15 cells, individually. (c) and (d) were the plasmids of interest transfect into HepG2 cells (c) and L02 cells (d), then the proliferation was measured at 12 h, 24 h, 36 h, 48 h, and 72 h, individually. Empty vector was set as the negative control. The dot, square, and triangle referred to IRAK4 428Ala (WT), IRAK4 428Thr (A428T), and control, respectively. represented that the comparison between IRAK4 428Ala and IRAK4 428Thr was statistically significant by Student’s -test, at .

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