Protein Kinase G Induces an Immune Response in Cows Exposed to Mycobacterium avium Subsp. paratuberculosis

Figure 3

PknG is secreted by MAP during infection. Fluorescence microscopy of infected THP-1 cells. THP-1 cells were infected with rhodamine 6G-labelled bacteria. Samples were obtained at 24, 48, and 72 h after infection and permeabilized with primary and secondary antibodies as described. PknG secretion was observed using fluorescence microscopy, where yellow indicates colocalization of the bacterium (in red) and the Alexa 488-anti-PknG antibodies (in green). Numbers on the left indicate hours. K, killed bacteria. White squares indicate phagosomes inside macrophages.