Identification of IL-28B Genotype Modification in Hepatocytes after Living Donor Liver Transplantation by Laser Capture Microdissection and Pyrosequencing Analysis

<table class="figure-group"><tr class="fig-image" id="a"><td><object data="https://static.hindawi.com/articles/bmri/volume-2018/1826140/figures/1826140.fig.004a.svgz" name="1826140.fig.004a" type="image/svg+xml"></object></td></tr><tr class="fig-caption"><td><b>(a) </b>Pyrosequencing analysis of the IL-28B rs8099917 single-nucleotide polymorphism in liver graft-isolated hepatocytes revealed the GT genotype, with a 14% G frequency and 86% T frequency. The analysis was performed using the PyroMark Q24 System (Qiagen) with Assay Design Software, version 2.0</td></tr><tr class="fig-image" id="b"><td><object data="https://static.hindawi.com/articles/bmri/volume-2018/1826140/figures/1826140.fig.004b.svgz" name="1826140.fig.004b" type="image/svg+xml"></object></td></tr><tr class="fig-caption"><td><b>(b) </b>Pyrosequencing analysis of the IL-28B SNP rs8099917 in serum from a recipient on preoperative day 0 (POD0) before living donor liver transplantation revealed the TT genotype, with a G frequency of only 3% and a T frequency of almost 97%</td></tr></table>

BioMed Research International

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Figure 4

Figure 4: Identification of IL-28B Genotype Modification in Hepatocytes after Living Donor Liver Transplantation by Laser Capture Microdissection and Pyrosequencing Analysis 

Figure 4 | Identification of IL-28B Genotype Modification in Hepatocytes after Living Donor Liver Transplantation by Laser Capture Microdissection and Pyrosequencing Analysis 