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BioMed Research International
Volume 2018, Article ID 1942451, 11 pages
https://doi.org/10.1155/2018/1942451
Research Article

Analysis of In Vitro Cyto- and Genotoxicity of Barbatimão Extract on Human Keratinocytes and Fibroblasts

1Postgraduate Program of Pharmacology, Federal University of Santa Maria, Santa Maria, RS, Brazil
2Postgraduate Program of Gerontology, Federal University of Santa Maria, Santa Maria, RS, Brazil
3Phytochemical Research Laboratory, Department of Industrial Pharmacy, Federal University of Santa Maria, Santa Maria, RS, Brazil
4Third Age Open University, University of Amazonas State, Manaus, AM, Brazil
5Brazilian Lutheran University (ULBRA), Santa Maria, RS, Brazil

Correspondence should be addressed to Ivana B. Mânica da Cruz; moc.liamg@msfu.anavi

Received 30 April 2018; Revised 24 August 2018; Accepted 13 September 2018; Published 8 October 2018

Guest Editor: Francesco Facchiano

Copyright © 2018 Neida L. Pellenz et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. M. Działo, J. Mierziak, U. Korzun, M. Preisner, J. Szopa, and A. Kulma, “The potential of plant phenolics in prevention and therapy of skin disorders,” International Journal of Molecular Sciences, vol. 17, no. 1, pp. 1–9, 2016. View at Google Scholar · View at Scopus
  2. L. M. Ricardo, B. M. Dias, F. L. B. Mügge, V. V. Leite, and M. G. L. Brandão, “Evidence of traditionality of Brazilian medicinal plants: The case studies of Stryphnodendron adstringens (Mart.) Coville (barbatimão) barks and Copaifera spp. (copaíba) oleoresin in wound healing,” Journal of Ethnopharmacology, vol. 219, pp. 319–336, 2018. View at Publisher · View at Google Scholar · View at Scopus
  3. J. Sousa, N. Pedroso, L. Borges, G. A., J. Paula, and E. Conceicao, “Optimization of Ultrasound-assisted extraction of polyphenols, tannins and epigallocatechin gallate from barks of Stryphnodendron adstringens (Mart.) Coville bark extracts,” Pharmacognosy Magazine, vol. 10, no. 38, p. 318, 2014. View at Publisher · View at Google Scholar
  4. A. C. Isler, G. C. Lopes, M. L. C. Cardoso, J. C. P. De Mello, and L. C. Marques, “Development and validation of a LC-method for the determination of phenols in a pharmaceutical formulation containing extracts from stryphnodendron adstringens,” Química Nova, vol. 33, no. 5, pp. 1126–1129, 2010. View at Publisher · View at Google Scholar · View at Scopus
  5. T. H. L. Bernardo, R. C. Sales Santos Veríssimo, V. Alvino et al., “Antimicrobial Analysis of an Antiseptic Made from Ethanol Crude Extracts of P. granatum and E. uniflora in Wistar Rats against Staphylococcus aureus and Staphylococcus epidermidis,” The Scientific World Journal, vol. 2015, Article ID 751791, 7 pages, 2015. View at Publisher · View at Google Scholar
  6. B. F. de Santana, R. A. Voeks, and L. S. Funch, “Ethnomedicinal survey of a maroon community in Brazil's Atlantic tropical forest,” Journal of Ethnopharmacology, vol. 181, pp. 37–49, 2016. View at Publisher · View at Google Scholar · View at Scopus
  7. S. C. G. Pinto, F. G. Bueno, G. P. Panizzon et al., “Stryphnodendron adstringens: Clarifying Wound Healing in Streptozotocin-Induced Diabetic Rats,” Planta Medica, vol. 81, no. 12-13, pp. 1090–1096, 2015. View at Publisher · View at Google Scholar · View at Scopus
  8. L. A. F. Silva, M. I. de Moura, C. E. Dambros, S. L. R. Freitas, L. A. Souza, and M. P. Abreu, “Stryphnodendron adstringens extract associated with the hooves trimming surgical procedure for the treatment of bovine digital dermatitis,” Tropical Animal Health and Production, vol. 45, no. 5, pp. 1177–1181, 2013. View at Publisher · View at Google Scholar · View at Scopus
  9. L. Hernandes, L. M. da Silva Pereira, F. Palazzo, and J. C. P. de Mello, “Wound-healing evaluation of ointment from Stryphnodendron adstringens (barbatimão) in rat skin,” Brazilian Journal of Pharmaceutical Sciences, vol. 46, no. 3, pp. 431–436, 2010. View at Publisher · View at Google Scholar · View at Scopus
  10. B. O. Henriques, O. Corrïa, E. P. C. Azevedo et al., “In vitro TNF- a inhibitory activity of brazilian plants and anti-inflammatory effect of Stryphnodendron adstringens in an acute arthritis model,” Evidence-based Complementary and Alternative Medicine, vol. 2016, Article ID 9872598, 15 pages, 2016. View at Publisher · View at Google Scholar
  11. E. M. R. Pereira, R. T. Gomes, N. R. Freire, E. G. Aguiar, M. D. G. L. Brandão, and V. R. Santos, “In vitro antimicrobial activity of Brazilian medicinal plant extracts against pathogenic microorganisms of interest to dentistry,” Planta Medica, vol. 77, no. 4, pp. 401–404, 2011. View at Publisher · View at Google Scholar · View at Scopus
  12. A. T. Morey, F. C. de Souza, J. P. Santos et al., “Antifungal activity of condensed tannins from stryphnodendron adstringens: Effect on Candida tropicalis growth and adhesion properties,” Current Pharmaceutical Biotechnology, vol. 17, no. 4, pp. 365–375, 2016. View at Publisher · View at Google Scholar · View at Scopus
  13. G. M. Lanchoti Fiori, A. L. Fachin, V. S. Correa et al., “Antimicrobial Activity and Rates of Tannins in Stryphnodendron adstringens Mart. Accessions Collected in the Brazilian Cerrado,” American Journal of Plant Sciences, vol. 04, no. 11, pp. 2193–2198, 2013. View at Publisher · View at Google Scholar
  14. M. A. Costa, J. C. Palazzo De Mello, E. N. Kaneshima et al., “Acute and chronic toxicity of an aqueous fraction of the stem bark of stryphnodendron adstringens (Barbatimão) in rodents,” Evidence-Based Complementary and Alternative Medicine, vol. 2013, Article ID 841580, 9 pages, 2013. View at Publisher · View at Google Scholar · View at Scopus
  15. M. A. Costa, K. Ishida, V. Kaplum et al., “Safety evaluation of proanthocyanidin polymer-rich fraction obtained from stem bark of Stryphnodendron adstringens (BARBATIMO) for use as a pharmacological agent,” Regulatory Toxicology and Pharmacology, vol. 58, no. 2, pp. 330–335, 2010. View at Publisher · View at Google Scholar · View at Scopus
  16. M. A. Rebecca, E. L. Ishii-Iwamoto, R. Grespan et al., “Toxicological studies on Stryphnodendron adstringens,” Journal of Ethnopharmacology, vol. 83, no. 1-2, pp. 101–104, 2002. View at Publisher · View at Google Scholar · View at Scopus
  17. P. Cintra, O. Malaspina, and O. C. Bueno, “Toxicity of barbatimão to Apis mellifera and Scaptotrigona postica, under laboratory conditions,” Journal of Apicultural Research, vol. 42, no. 1-2, pp. 9–12, 2003. View at Publisher · View at Google Scholar · View at Scopus
  18. C. Griesinger, “Validation of Alternative in vitro Methods to Animal Testing: Concepts, Challenges, Processes and Tools,” Advances in Experimental Medicine and Biology, vol. 1, pp. 1–9, 2016. View at Google Scholar
  19. F. C. Cadoná, M. F. Manica-Cattani, A. K. Machado et al., “Genomodifier capacity assay: A non-cell test using dsDNA molecules to evaluate the genotoxic/genoprotective properties of chemical compounds,” Analytical Methods, vol. 6, no. 21, pp. 8559–8568, 2014. View at Publisher · View at Google Scholar · View at Scopus
  20. N. Dabrowska and A. Wiczkowski, “Analytics of oxidative stress markers in the early diagnosis of oxygen DNA damage,” Advances in Clinical and Experimental Medicine, vol. 26, no. 1, pp. 155–166, 2017. View at Publisher · View at Google Scholar · View at Scopus
  21. L. Bergandi, E. Mungo, R. Morone, B. Rolando, and S. Doublier, “Hyperglycemia Promotes Chemoresistance Through the Reduction of the Mitochondrial DNA Damage, the Bax/Bcl-2 and Bax/Bcl-XL Ratio, and the Cells in Sub-G1 Phase Due to Antitumoral Drugs Induced-Cytotoxicity in Human Colon Adenocarcinoma,” Cells, vol. 13, p. 866, 2018. View at Google Scholar
  22. V. F. Azzolin, F. Barbisan, L. S. Lenz et al., “Effects of Pyridostigmine bromide on SH-SY5Y cells: An in vitro neuroblastoma neurotoxicity model,” Mutation Research - Genetic Toxicology and Environmental Mutagenesis, vol. 8, pp. 1–10, 2018. View at Publisher · View at Google Scholar
  23. F. Barbisan, J. De Rosso Motta, A. Trott et al., “Methotrexate-related response on human peripheral blood mononuclear cells may be modulated by the Ala16Val-SOD2 gene polymorphism,” PLoS ONE, vol. 9, no. 10, Article ID e107299, 2014. View at Publisher · View at Google Scholar · View at Scopus
  24. J. E. C. Betoni, R. P. Mantovani, L. N. Barbosa, L. C. di Stasi, and A. Fernandes Jr., “Synergism between plant extract and antimicrobial drugs used on Staphylococcus aureus diseases,” Memórias do Instituto Oswaldo Cruz, vol. 101, no. 4, pp. 387–390, 2006. View at Publisher · View at Google Scholar · View at Scopus
  25. E. da Silva Brum, L. da Rosa Moreira, A. R. H. da Silva et al., “Tabernaemontana catharinensis ethyl acetate fraction presents antinociceptive activity without causing toxicological effects in mice,” Journal of Ethnopharmacology, vol. 191, pp. 115–124, 2016. View at Publisher · View at Google Scholar · View at Scopus
  26. X.-C. Xie, Y. Cao, X. Yang, Q.-H. Xu, W. Wei, and M. Wang, “Relaxin Attenuates Contrast-Induced Human Proximal Tubular Epithelial Cell Apoptosis by Activation of the PI3K/Akt Signaling Pathway In Vitro,” BioMed Research International, vol. 2017, Article ID 2869405, 7 pages, 2017. View at Publisher · View at Google Scholar
  27. V. F. Azzolin, F. C. Cadoná, A. K. Machado et al., “Superoxide-hydrogen peroxide imbalance interferes with colorectal cancer cells viability, proliferation and oxaliplatin response,” Toxicology in Vitro, vol. 32, pp. 8–15, 2016. View at Publisher · View at Google Scholar · View at Scopus
  28. Brasil. Farmacopeia Brasileira, vol. 2, Agência Nacional de Vigilância Sanitária. Brasília: Anvisa, 546p. 2010.
  29. S. C. Santos, W. F. Costa, J. P. Ribeiro et al., “Tannin composition of barbatimão species,” Fitoterapia, vol. 73, no. 4, pp. 292–299, 2002. View at Publisher · View at Google Scholar · View at Scopus
  30. T. Hatahet, M. Morille, A. Hommoss, J. M. Devoisselle, R. H. Müller, and S. Bégu, “Quercetin topical application, from conventional dosage forms to nanodosage forms,” European Journal of Pharmaceutics and Biopharmaceutics, vol. 108, pp. 41–53, 2016. View at Publisher · View at Google Scholar · View at Scopus
  31. R. Sarıkaya, K. Erciyas, M. I. Kara, U. Sezer, A. F. Erciyas, and S. Ay, “Evaluation of genotoxic and antigenotoxic effects of boron by the somatic mutation and recombination test (SMART) on Drosophila,” Drug and Chemical Toxicology, vol. 39, no. 4, pp. 400–406, 2016. View at Publisher · View at Google Scholar · View at Scopus
  32. J. B. Vilar, M. I. P. D'Oliveira, S. D. C. Santos, and L. C. Chen, “Cytotoxic and genotoxic investigation on barbatimão [Stryphnodendron adstringens (Mart.) Coville, 1910] extract,” Brazilian Journal of Pharmaceutical Sciences, vol. 46, no. 4, pp. 687–694, 2010. View at Publisher · View at Google Scholar · View at Scopus
  33. J. Chayapong, H. Madhyastha, R. Madhyastha et al., “Arsenic trioxide induces ROS activity and DNA damage, leading to G0/G1 extension in skin fibroblasts through the ATM-ATR-associated Chk pathway,” Environmental Science and Pollution Research, vol. 24, no. 6, pp. 5316–5325, 2017. View at Publisher · View at Google Scholar · View at Scopus
  34. E. A. Prokhorova, A. V. Zamaraev, G. S. Kopeina, B. Zhivotovsky, and I. N. Lavrik, “Role of the nucleus in apoptosis: Signaling and execution,” Cellular and Molecular Life Sciences, vol. 72, no. 23, pp. 4593–4612, 2015. View at Publisher · View at Google Scholar · View at Scopus