F806 Suppresses the Invasion and Metastasis of Esophageal Squamous Cell Carcinoma via Downregulating F-Actin Assembly-Related Rho Family Proteins

Figure 4

The inhibition of F806 on expression and activity of CDC42, RHOA, and RAC1. (a) Activity and expression analysis of CDC42 were detected by a Rho GTPase activation assay kit. (b) The expression of RHOA and RAC1 was detected by Western blot. (c) Western blots analysis of flag-RHOA and flag-RAC1 expression in ESCC cells after pretreatment with MG132 and then added F806. (d) The colocalization of actin filament and focal adhesion marker Paxillin was analyzed by immunofluorescent staining in EC109 and KYSE510 cells. Scale bar = 10 μm.

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