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BioMed Research International
Volume 2018 (2018), Article ID 2543210, 9 pages
Research Article

Fermentation Conditions and Media Optimization for Isocitric Acid Production from Ethanol by Yarrowia lipolytica

1G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pr-t Nauki 5, P.O. Box 142290, Pushchino, Moscow, Russia
2Peoples’ Friendship University of Russia (RUDN University), Miklukho-Maklaya Str. 6, P.O. Box 117198, Moscow, Russia
3Moscow Technological University (MIREA), Vernadskogo Pr. 78, P.O. Box 119454, Moscow, Russia

Correspondence should be addressed to Svetlana V. Kamzolova

Received 29 September 2017; Revised 18 December 2017; Accepted 10 January 2018; Published 7 February 2018

Academic Editor: Isabelle Chevalot

Copyright © 2018 Svetlana V. Kamzolova et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Isocitric acid exists in the form of four stereoisomers, of which only the threo-Ds-form (ICA) is a natural active compound, an intermediate of Krebs cycle, and suitable for nutritional and pharmaceutical use. In this paper, we propose a method for ICA production from ethanol by yeast Yarrowia lipolytica. The effects of temperature, pH of the medium, and aeration on the growth of the producer Y. lipolytica VKM Y-2373 and synthesis of ICA were studied. An optimal fermentation regime, which ensures a good growth of the producer and directed synthesis of the target product, was determined. The producer is advised to carry out cultivation at 29°C and various pH of the medium and the oxygen concentration (pH 5 and pO2 20–25% (of saturation) during the growth period and pH 6 and pO2 50–55% (of saturation) during the acid formation) on a nutrient medium containing an increased content of zinc (0.6 mg/L), iron (1.2 mg/L), and 30 mM itaconic acid (inhibitor of isocitrate lyase—the key enzyme of ICA metabolism) should also be introduced into the nutrition medium. Such fermentation production mode provides 90.5 g/L ICA with process selectivity of 80%, mass yield () of 0.77 g/g, and energy yield () of 0.278 g/g.