Research Article

-Hederin Arrests Cell Cycle at G2/M Checkpoint and Promotes Mitochondrial Apoptosis by Blocking Nuclear Factor-B Signaling in Colon Cancer Cells

Figure 4

α-Hederin inhibits NF-κB signaling in IL-6-stimulated SW620 cells. SW620 cells were treated with vehicle, IL-6, and/or α-hederin at indicated concentrations for 24 h. (a) Western blot analysis of nuclear abundance of NF-κB with quantification. Significance: P<0.01 versus control, #P<0.05 versus IL-6, ##P<0.01 versus IL-6. (b) Immunofluorescence analyses of the nuclear translocation NF-κB in SW620 cells treated with α-hederin at 10 μg/ml and/or IL-6 at 6.25 ng/ml (200 x magnification). (c) Western blot analysis of protein abundance of p-IκBα, IκBα, p-IKKα, and IKKα with quantification. Significance: P<0.05 versus control, P<0.01 versus control, #P<0.05 versus IL-6, ##P<0.01 versus IL-6.