Identification of Feline Interferon Regulatory Factor 1 as an Efficient Antiviral Factor against the Replication of Feline Calicivirus and Other Feline Viruses

Figure 2

Fe-IRF1 inhibits FCV replication. (a, b, c) CRFK cells were transfected with 500 ng/well of the pMyc-IRF1. At 24 h posttransfection, cells were infected with FCV F9 at an MOI of 0.01 for 24 h. The relative genome RNA (a), viral yields (b), and the expression of VP1 (c) of FCV were detected. (d) CRFK cells were transfected with 500 ng/well of the p3×Flag-IFN-β or p3×Flag-CMV-10. The mRNA levels of endogenous IRF1 were detected by qRT-PCR 24 hours posttransfection. (e) p3×Flag-IRF1 (50, 100, 200, 400, 1000, and 2000 ng) were transfected into cells. At 24 h posttransfection, cells were infected with FCV F9 at an MOI of 0.01. At 24 h posttransfection, the viral yields of each sample were determined. (f) The cells were transfected with 500 ng/well of the pMyc-IRF1 plasmid or empty vector for 24 h; then FCV with an MOI of 0.01 was inoculated. At 12 h, 24 h, 36 h, 48 h, 60 h, and 72 h postinoculation, the viral yields of each sample were determined. Error bars represent standard deviations and each sample was run in triplicate.: P<0.05; : P<0.01.

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