Identification of Feline Interferon Regulatory Factor 1 as an Efficient Antiviral Factor against the Replication of Feline Calicivirus and Other Feline Viruses

Figure 3

Fe-IRF1 is located in the nucleus and can activate the IFN-β pathway. (a) The N terminus sequences from 1 to 125 amino acid of human, mouse, rat, pig, bovine, dog, and cat IRF1 were aligned using MEGA5. (b) CRFK cells were transfected with pEGFP-IRF1 and subjected to confocal microscopy. (c, d) CRFK cells were cotransfected with 0.25 μg/well of the reporter plasmid pIFN-β-Luc (c) or pISRE-TA-Luc (d) and 0.25 μg/well of p3×Flag-IRF1 or empty vector, along with 0.02 μg /well of pRL-TK plasmid. Luciferase assays were performed at 24 h posttransfection. The SeV infection group serves as a positive control. The data shown represent the mean ± SD for three independent experiments. : P<0.05; : P<0.01.

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