Research Article
A Comparative Study of Three Detection Techniques for Leifsonia xyli Subsp. xyli, the Causal Pathogen of Sugarcane Ratoon Stunting Disease
Figure 1
The specificity of detecting Leifsonia xyli subsp. xyli, the causal pathogen of sugarcane ratoon disease. (a) Agarose gel electropherograms of PCR amplification products. (b) Fluorescent color change of PCR amplification products. Lane M: 100 bp marker; Lane 1: ddH2O; Lane 2: 20 ng/μL DNA sample extracted from Lxx-free sugarcane juice; Lane 3: 20 ng/μL Leifsonia poae plasmid; Lane 4: 20 ng/μL Leifsonia ginsengi plasmid; Lane 5: 20 ng/μL DNA sample from Lxx-infected sugarcane juice; Lane 6: 20 ng/μL Lxx-pMD18-T plasmid.
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