A Comparative Study of Three Detection Techniques for Leifsonia xyli Subsp. xyli, the Causal Pathogen of Sugarcane Ratoon Stunting Disease

Figure 3

Agarose gel electropherograms of conventional PCR products of Lxx-pMD18-T plasmids. Lane M: 100 bp molecular marker; Lane 1: ddH₂O; Lane 2: 20 ng/μL negative DNA extracted from Lxx-free juice; Lanes 3 to 12: 10-fold serial dilutions of Lxx-pMD18-T plasmid (10⁰ to 10⁻¹⁰, 1.0 ng/μL to 1.0 × 10⁻¹⁰ ng/μL). The red arrow points to the limiting detection concentration of Lxx-pMD18-T plasmid.