Research Article

A Comparative Study of Three Detection Techniques for Leifsonia xyli Subsp. xyli, the Causal Pathogen of Sugarcane Ratoon Stunting Disease

Figure 5

Detection of products amplified by LAMP reactions with DNA extracted from Lxx-infected sugarcane juice based on color changes. (a) Results of experiments conducted on 04/25/2013; (b) results of experiments conducted on 06/28/2013. Lane 1: ddH2O; Lane 2: 20 ng/μL DNA sample extracted from Lxx-free sugarcane juice; Lanes 3 to 10: 10-fold serial dilutions of DNA (100 to 10−7, 4.0 ng/μL to 4.0 × 10−7 ng/μL) extracted from Lxx-infected sugarcane juice.
(a)
(b)