A Comparative Study of Three Detection Techniques for Leifsonia xyli Subsp. xyli, the Causal Pathogen of Sugarcane Ratoon Stunting Disease

Table 1

Sequences of PCR, LAMP, and RT-qPCR primers used for Lxx detection.

Primer	Sequence (5′to 3′)
Cxx	CCGAAGTGAGCAGATTGACC
Cxx2	ACCCTGTGTTGTTTTCAACG
F	ACATCGGTACGACTGGGT
B3	TGGCCGACCAAAAAAGGT
FIP	GGCGTACTAAGTTCGAGCCGTT-GGTCAGCTCATGGGTGGA
BIP	CCTCGCACATGCACGCTGTT-CTCAGCGTCTTGAAGACACA
LF	CTCCGCACCAATGTCAATGT
LP	CTGAGGGACCGGACCTCATC
Lxx82	TTCAACGCAGAAATTGTCCAGG
Lxx22R	CAAGCAGGCGTACTAAGTTCGA

Cxx1/Cxx2 primers were according to Pan et al. [20].
F3/B3 & FIP/BIP primers were according to Liu et al. [43].
Lxx82F/Lxx22R primers were according to Grisham et al. [33].