A Comparative Study of Three Detection Techniques for Leifsonia xyli Subsp. xyli, the Causal Pathogen of Sugarcane Ratoon Stunting Disease

Table 3

Ct values of RT-qPCR using serial dilution of Lxx-pMD18-T plasmid as templates.

Sample	2013.5.		2013.6.2
		Mean Ct	Ct	Mean Ct
Blank (ddH2O)	—	—	—	—
Negative plasmid	—	—	—	—
10^0  Lxx-pMD18-T plasmid	8.3205; 8.4208; 8.2091	8.3168	8.5207; 7.8205; 7.947	8.096
10^−1  Lxx-pMD18-T plasmid	11.5595; 11.4471; 11.3005	11.4357	11.2019; 11.4752; 10.9927	11.223
10^−2  Lxx-pMD18-T plasmid	13.515; 13.4772; 13.5749	13.5224	13.62; 13.7713; 13.5248	13.639
10^−3  Lxx-pMD18-T plasmid	14.7635; 14.6707; 14.6183	14.6842	16.1848; 16.1488; 16.2099	16.121
10^−4  Lxx-pMD18-T plasmid	18.0854; 18.1271; 18.0325	18.0817	20.161; 20.0562; 19.7764	19.998
10^−5  Lxx-pMD18-T plasmid	23.0183; 23.3196; —	23.169	24.3614; 24.4379; 23.9269	24.242
10^−6  Lxx-pMD18-T plasmid	25.0384; 24.8664; 24.7652	24.8900	28.0877; 28.1149; 27.6601	27.955
10^−7  Lxx-pMD18-T plasmid	29.1153; 19.8728; 29.2228	29.0703	30.9799; 30.3203; —	30.6501
10^−8  Lxx-pMD18-T plasmid	33.412; 32.8918; —	33.1519	34.906; 32.892; 33.412	33.737
10^−9  Lxx-pMD18-T plasmid	—	—	—	—
10^−10  Lxx-pMD18-T plasmid	—	—	—	—

Results of experiments conducted on 04/25/2013.
Results of experiments conducted on 06/26/2013.
“Ct” means cycle threshold.
“—” means absence detection of Lxx.